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Amir SaberiUpdate name and description
f71c237•1 month ago
AID-BE3
8439 bp
All features (28)
M13 rev
Type:primer_bind
Length:17 bp
Location:5794 - 5811
6xHis
Type:cds
Length:18 bp
Location:5453 - 5471
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:5499 - 5724
SV40 NLS
Type:cds
Length:21 bp
Location:5424 - 5445
CMV promoter
Type:promoter
Length:204 bp
Location:131 - 335
CMV enhancer
Type:enhancer
Length:-8059 bp
Location:8190 - 131
T7 promoter
Type:promoter
Length:19 bp
Location:376 - 395
Kozak sequence
Type:regulatory
Length:10 bp
Location:402 - 412
ori
Type:origin
Length:589 bp
Location:6196 - 6785
lac operator
Type:protein_bind
Length:17 bp
Location:5818 - 5835
CAP binding site
Type:protein_bind
Length:22 bp
Location:5887 - 5909
lac promoter
Type:promoter
Length:31 bp
Location:5842 - 5873
UGI
Type:cds
Length:249 bp
Location:5163 - 5412
AID
Type:cds
Length:594 bp
Location:408 - 1002
Cas9 VQR
Type:cds
Length:4101 bp
Location:1050 - 5151
Cas9(D10A)
Type:cds
Length:4101 bp
Location:1050 - 5151
AmpR
Type:cds
Length:861 bp
Location:6955 - 7816
AmpR promoter
Type:promoter
Length:105 bp
Location:7816 - 7921
Amp-R
Type:primer_bind
Length:20 bp
Location:7577 - 7597
BGH-rev
Type:primer_bind
Length:18 bp
Location:5492 - 5510
CMV-F
Type:primer_bind
Length:21 bp
Location:284 - 305
L4440 (site 1)
Type:primer_bind
Length:18 bp
Location:6024 - 6042
L4440 (site 2)
Type:primer_bind
Length:10 bp
Location:8069 - 8079
M13 Reverse
Type:primer_bind
Length:17 bp
Location:5793 - 5810
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:5806 - 5829
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:6275 - 6295
pRS-marker
Type:primer_bind
Length:20 bp
Location:7998 - 8018
T7
Type:primer_bind
Length:20 bp
Location:375 - 395
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 5794 - 5811 | 17 bp | |
6xHis | cds | → right | 5453 - 5471 | 18 bp | |
bGH poly(A) signal | polya_signal | → right | 5499 - 5724 | 225 bp | |
SV40 NLS | cds | → right | 5424 - 5445 | 21 bp | |
CMV promoter | promoter | → right | 131 - 335 | 204 bp | |
CMV enhancer | enhancer | → right | 8190 - 131 | -8059 bp | |
T7 promoter | promoter | → right | 376 - 395 | 19 bp | |
Kozak sequence | regulatory | → right | 402 - 412 | 10 bp | |
ori | origin | ← left | 6196 - 6785 | 589 bp | |
lac operator | protein_bind | → right | 5818 - 5835 | 17 bp | |
CAP binding site | protein_bind | → right | 5887 - 5909 | 22 bp | |
lac promoter | promoter | ← left | 5842 - 5873 | 31 bp | |
UGI | cds | → right | 5163 - 5412 | 249 bp | |
AID | cds | → right | 408 - 1002 | 594 bp | |
Cas9 VQR | cds | → right | 1050 - 5151 | 4101 bp | |
Cas9(D10A) | cds | → right | 1050 - 5151 | 4101 bp | |
AmpR | cds | ← left | 6955 - 7816 | 861 bp | |
AmpR promoter | promoter | ← left | 7816 - 7921 | 105 bp | |
Amp-R | primer_bind | → right | 7577 - 7597 | 20 bp | |
BGH-rev | primer_bind | ← left | 5492 - 5510 | 18 bp | |
CMV-F | primer_bind | → right | 284 - 305 | 21 bp | |
L4440 (site 1) | primer_bind | ← left | 6024 - 6042 | 18 bp | |
L4440 (site 2) | primer_bind | → right | 8069 - 8079 | 10 bp | |
M13 Reverse | primer_bind | ← left | 5793 - 5810 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 5806 - 5829 | 23 bp | |
pBR322ori-F | primer_bind | ← left | 6275 - 6295 | 20 bp | |
pRS-marker | primer_bind | ← left | 7998 - 8018 | 20 bp | |
T7 | primer_bind | → right | 375 - 395 | 20 bp |