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A
Amir SaberiUpdate name and description
f71c237•1 month ago
Cbh_v5 AAV-CBE C-terminal
7658 bp
All features (36)
M13 rev
Type:primer_bind
Length:17 bp
Location:2873 - 2890
M13 fwd
Type:primer_bind
Length:17 bp
Location:124 - 141
U6 promoter
Type:promoter
Length:249 bp
Location:7362 - 7611
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:7027 - 7252
hybrid intron
Type:intron
Length:228 bp
Location:3633 - 3861
SV40 NLS
Type:cds
Length:21 bp
Location:6746 - 6767
SV40 NLS
Type:cds
Length:21 bp
Location:3914 - 3935
chicken β-actin promoter
Type:promoter
Length:278 bp
Location:3355 - 3633
CMV enhancer
Type:enhancer
Length:286 bp
Location:3068 - 3354
f1 ori
Type:origin
Length:456 bp
Location:282 - 738
ori
Type:origin
Length:589 bp
Location:1899 - 2488
lac operator
Type:protein_bind
Length:17 bp
Location:2849 - 2866
CAP binding site
Type:protein_bind
Length:22 bp
Location:2775 - 2797
lac promoter
Type:promoter
Length:31 bp
Location:2811 - 2842
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:7265 - 7341
UGI
Type:cds
Length:249 bp
Location:6455 - 6704
Cas9(C)
Type:cds
Length:2385 bp
Location:4040 - 6425
AmpR
Type:cds
Length:861 bp
Location:868 - 1729
AmpR promoter
Type:promoter
Length:105 bp
Location:763 - 868
AAV2 ITR
Type:repeat_region
Length:-7528 bp
Location:7634 - 106
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:2916 - 3046
Amp-R
Type:primer_bind
Length:20 bp
Location:1085 - 1105
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:578 - 600
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:2239 - 2249
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:5197 - 5207
F1ori-R
Type:primer_bind
Length:20 bp
Location:368 - 388
hU6-F (site 1)
Type:primer_bind
Length:10 bp
Location:2562 - 2572
hU6-F (site 2)
Type:primer_bind
Length:21 bp
Location:7589 - 7610
LKO.1 5' (site 1)
Type:primer_bind
Length:10 bp
Location:6887 - 6897
LKO.1 5' (site 2)
Type:primer_bind
Length:20 bp
Location:7419 - 7439
M13 Forward
Type:primer_bind
Length:18 bp
Location:123 - 141
M13 Reverse
Type:primer_bind
Length:17 bp
Location:2872 - 2889
M13/pUC Forward
Type:primer_bind
Length:23 bp
Location:132 - 155
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:2853 - 2876
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:2387 - 2407
WPRE-R
Type:primer_bind
Length:21 bp
Location:6830 - 6851
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | → right | 2873 - 2890 | 17 bp | |
M13 fwd | primer_bind | ← left | 124 - 141 | 17 bp | |
U6 promoter | promoter | ← left | 7362 - 7611 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 7027 - 7252 | 225 bp | |
hybrid intron | intron | → right | 3633 - 3861 | 228 bp | |
SV40 NLS | cds | → right | 6746 - 6767 | 21 bp | |
SV40 NLS | cds | → right | 3914 - 3935 | 21 bp | |
chicken β-actin promoter | promoter | → right | 3355 - 3633 | 278 bp | |
CMV enhancer | enhancer | → right | 3068 - 3354 | 286 bp | |
f1 ori | origin | → right | 282 - 738 | 456 bp | |
ori | origin | → right | 1899 - 2488 | 589 bp | |
lac operator | protein_bind | → right | 2849 - 2866 | 17 bp | |
CAP binding site | protein_bind | → right | 2775 - 2797 | 22 bp | |
lac promoter | promoter | → right | 2811 - 2842 | 31 bp | |
gRNA scaffold | misc_rna | ← left | 7265 - 7341 | 76 bp | |
UGI | cds | → right | 6455 - 6704 | 249 bp | |
Cas9(C) | cds | → right | 4040 - 6425 | 2385 bp | |
AmpR | cds | → right | 868 - 1729 | 861 bp | |
AmpR promoter | promoter | → right | 763 - 868 | 105 bp | |
AAV2 ITR | repeat_region | → right | 7634 - 106 | -7528 bp | |
AAV2 ITR | repeat_region | → right | 2916 - 3046 | 130 bp | |
Amp-R | primer_bind | ← left | 1085 - 1105 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 578 - 600 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 2239 - 2249 | 10 bp | |
F1ori-F (site 3) | primer_bind | → right | 5197 - 5207 | 10 bp | |
F1ori-R | primer_bind | ← left | 368 - 388 | 20 bp | |
hU6-F (site 1) | primer_bind | → right | 2562 - 2572 | 10 bp | |
hU6-F (site 2) | primer_bind | ← left | 7589 - 7610 | 21 bp | |
LKO.1 5' (site 1) | primer_bind | → right | 6887 - 6897 | 10 bp | |
LKO.1 5' (site 2) | primer_bind | ← left | 7419 - 7439 | 20 bp | |
M13 Forward | primer_bind | ← left | 123 - 141 | 18 bp | |
M13 Reverse | primer_bind | → right | 2872 - 2889 | 17 bp | |
M13/pUC Forward | primer_bind | ← left | 132 - 155 | 23 bp | |
M13/pUC Reverse | primer_bind | → right | 2853 - 2876 | 23 bp | |
pBR322ori-F | primer_bind | → right | 2387 - 2407 | 20 bp | |
WPRE-R | primer_bind | ← left | 6830 - 6851 | 21 bp |