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Amir SaberiUpdate name and description
f71c237•1 month ago
pAAV-ABE-NT-sgRNA
7052 bp
All features (22)
SK primer
Type:primer_bind
Length:17 bp
Location:849 - 866
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:4314 - 4455
U6 promoter
Type:promoter
Length:249 bp
Location:144 - 393
SV40 NLS
Type:cds
Length:21 bp
Location:888 - 909
f1 ori
Type:origin
Length:456 bp
Location:4529 - 4985
ori
Type:origin
Length:589 bp
Location:6402 - 6991
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:414 - 490
AmpR
Type:cds
Length:861 bp
Location:5371 - 6232
AmpR promoter
Type:promoter
Length:105 bp
Location:5266 - 5371
AAV2 ITR (alternate)
Type:repeat_region
Length:130 bp
Location:0 - 130
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:4314 - 4444
Amp-R
Type:primer_bind
Length:20 bp
Location:5588 - 5608
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:4825 - 4847
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:6742 - 6752
F1ori-R
Type:primer_bind
Length:20 bp
Location:4615 - 4635
hU6-F
Type:primer_bind
Length:21 bp
Location:143 - 164
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:314 - 334
pBluescriptSK
Type:primer_bind
Length:17 bp
Location:848 - 865
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:6890 - 6910
pBRforEco
Type:primer_bind
Length:19 bp
Location:5179 - 5198
pGEX 3'
Type:primer_bind
Length:23 bp
Location:5119 - 5142
pRS-marker
Type:primer_bind
Length:20 bp
Location:5000 - 5020
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
SK primer | primer_bind | → right | 849 - 866 | 17 bp | |
AAV2 ITR | repeat_region | → right | 4314 - 4455 | 141 bp | |
U6 promoter | promoter | → right | 144 - 393 | 249 bp | |
SV40 NLS | cds | → right | 888 - 909 | 21 bp | |
f1 ori | origin | → right | 4529 - 4985 | 456 bp | |
ori | origin | → right | 6402 - 6991 | 589 bp | |
gRNA scaffold | misc_rna | → right | 414 - 490 | 76 bp | |
AmpR | cds | → right | 5371 - 6232 | 861 bp | |
AmpR promoter | promoter | → right | 5266 - 5371 | 105 bp | |
AAV2 ITR (alternate) | repeat_region | → right | 0 - 130 | 130 bp | |
AAV2 ITR | repeat_region | → right | 4314 - 4444 | 130 bp | |
Amp-R | primer_bind | ← left | 5588 - 5608 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 4825 - 4847 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 6742 - 6752 | 10 bp | |
F1ori-R | primer_bind | ← left | 4615 - 4635 | 20 bp | |
hU6-F | primer_bind | → right | 143 - 164 | 21 bp | |
LKO.1 5' | primer_bind | → right | 314 - 334 | 20 bp | |
pBluescriptSK | primer_bind | → right | 848 - 865 | 17 bp | |
pBR322ori-F | primer_bind | → right | 6890 - 6910 | 20 bp | |
pBRforEco | primer_bind | ← left | 5179 - 5198 | 19 bp | |
pGEX 3' | primer_bind | → right | 5119 - 5142 | 23 bp | |
pRS-marker | primer_bind | ← left | 5000 - 5020 | 20 bp |