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Amir SaberiUpdate name and description
f71c237•1 month ago
pAAV-CASI-InteinC-NG C aa714-1368
7255 bp
All features (34)
M13 rev
Type:primer_bind
Length:17 bp
Location:7212 - 7229
M13 fwd
Type:primer_bind
Length:17 bp
Location:4463 - 4480
U6 promoter
Type:promoter
Length:249 bp
Location:3732 - 3981
β-globin poly(A) signal
Type:polya_signal
Length:56 bp
Location:3476 - 3532
SV40 NLS
Type:cds
Length:21 bp
Location:3441 - 3462
chicken β-actin promoter
Type:promoter
Length:278 bp
Location:521 - 799
CMV enhancer
Type:enhancer
Length:304 bp
Location:216 - 520
Kozak sequence
Type:regulatory
Length:10 bp
Location:1276 - 1286
Kozak sequence
Type:regulatory
Length:10 bp
Location:1272 - 1282
f1 ori
Type:origin
Length:456 bp
Location:4621 - 5077
ori
Type:origin
Length:589 bp
Location:6238 - 6827
lac operator
Type:protein_bind
Length:17 bp
Location:7188 - 7205
CAP binding site
Type:protein_bind
Length:22 bp
Location:7114 - 7136
lac promoter
Type:promoter
Length:31 bp
Location:7150 - 7181
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:3636 - 3712
AmpR
Type:cds
Length:861 bp
Location:5207 - 6068
AmpR promoter
Type:promoter
Length:105 bp
Location:5102 - 5207
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:4315 - 4445
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:11 - 141
Amp-R
Type:primer_bind
Length:20 bp
Location:5424 - 5444
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:2171 - 2181
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:4917 - 4939
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:6578 - 6588
F1ori-R
Type:primer_bind
Length:20 bp
Location:4707 - 4727
hU6-F (site 1)
Type:primer_bind
Length:21 bp
Location:3959 - 3980
hU6-F (site 2)
Type:primer_bind
Length:10 bp
Location:6901 - 6911
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:3789 - 3809
M13 Forward
Type:primer_bind
Length:18 bp
Location:4462 - 4480
M13 Reverse (site 1)
Type:primer_bind
Length:11 bp
Location:4203 - 4214
M13 Reverse (site 2)
Type:primer_bind
Length:17 bp
Location:7211 - 7228
M13/pUC Forward
Type:primer_bind
Length:23 bp
Location:4471 - 4494
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:7192 - 7215
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:6726 - 6746
rbglobpA-R
Type:primer_bind
Length:20 bp
Location:3529 - 3549
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | → right | 7212 - 7229 | 17 bp | |
M13 fwd | primer_bind | ← left | 4463 - 4480 | 17 bp | |
U6 promoter | promoter | ← left | 3732 - 3981 | 249 bp | |
β-globin poly(A) signal | polya_signal | → right | 3476 - 3532 | 56 bp | |
SV40 NLS | cds | → right | 3441 - 3462 | 21 bp | |
chicken β-actin promoter | promoter | → right | 521 - 799 | 278 bp | |
CMV enhancer | enhancer | → right | 216 - 520 | 304 bp | |
Kozak sequence | regulatory | → right | 1276 - 1286 | 10 bp | |
Kozak sequence | regulatory | → right | 1272 - 1282 | 10 bp | |
f1 ori | origin | → right | 4621 - 5077 | 456 bp | |
ori | origin | → right | 6238 - 6827 | 589 bp | |
lac operator | protein_bind | → right | 7188 - 7205 | 17 bp | |
CAP binding site | protein_bind | → right | 7114 - 7136 | 22 bp | |
lac promoter | promoter | → right | 7150 - 7181 | 31 bp | |
gRNA scaffold | misc_rna | ← left | 3636 - 3712 | 76 bp | |
AmpR | cds | → right | 5207 - 6068 | 861 bp | |
AmpR promoter | promoter | → right | 5102 - 5207 | 105 bp | |
AAV2 ITR | repeat_region | → right | 4315 - 4445 | 130 bp | |
AAV2 ITR | repeat_region | → right | 11 - 141 | 130 bp | |
Amp-R | primer_bind | ← left | 5424 - 5444 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 2171 - 2181 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 4917 - 4939 | 22 bp | |
F1ori-F (site 3) | primer_bind | ← left | 6578 - 6588 | 10 bp | |
F1ori-R | primer_bind | ← left | 4707 - 4727 | 20 bp | |
hU6-F (site 1) | primer_bind | ← left | 3959 - 3980 | 21 bp | |
hU6-F (site 2) | primer_bind | → right | 6901 - 6911 | 10 bp | |
LKO.1 5' | primer_bind | ← left | 3789 - 3809 | 20 bp | |
M13 Forward | primer_bind | ← left | 4462 - 4480 | 18 bp | |
M13 Reverse (site 1) | primer_bind | ← left | 4203 - 4214 | 11 bp | |
M13 Reverse (site 2) | primer_bind | → right | 7211 - 7228 | 17 bp | |
M13/pUC Forward | primer_bind | ← left | 4471 - 4494 | 23 bp | |
M13/pUC Reverse | primer_bind | → right | 7192 - 7215 | 23 bp | |
pBR322ori-F | primer_bind | → right | 6726 - 6746 | 20 bp | |
rbglobpA-R | primer_bind | ← left | 3529 - 3549 | 20 bp |