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Amir SaberiUpdate name and description
f71c237•1 month ago
pAAV-EFS-TadA8e-Sauri-U6-Lmna sgRNA
7897 bp
All features (28)
M13 rev
Type:primer_bind
Length:17 bp
Location:363 - 380
M13 fwd
Type:primer_bind
Length:17 bp
Location:5511 - 5528
U6 promoter
Type:promoter
Length:249 bp
Location:5091 - 5340
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:4736 - 4961
SV40 NLS
Type:cds
Length:21 bp
Location:4702 - 4723
SV40 NLS
Type:cds
Length:21 bp
Location:862 - 883
EF-1α core promoter
Type:promoter
Length:212 bp
Location:601 - 813
f1 ori
Type:origin
Length:456 bp
Location:5669 - 6125
ori
Type:origin
Length:589 bp
Location:7286 - 7875
lac operator
Type:protein_bind
Length:17 bp
Location:339 - 356
CAP binding site
Type:protein_bind
Length:22 bp
Location:265 - 287
lac promoter
Type:promoter
Length:31 bp
Location:301 - 332
AmpR
Type:cds
Length:861 bp
Location:6255 - 7116
AmpR promoter
Type:promoter
Length:105 bp
Location:6150 - 6255
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:5363 - 5493
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:406 - 536
Amp-R
Type:primer_bind
Length:20 bp
Location:6472 - 6492
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:5965 - 5987
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:7626 - 7636
F1ori-R
Type:primer_bind
Length:20 bp
Location:5755 - 5775
hU6-F (site 1)
Type:primer_bind
Length:10 bp
Location:52 - 62
hU6-F (site 2)
Type:primer_bind
Length:21 bp
Location:5318 - 5339
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:5148 - 5168
M13 Forward
Type:primer_bind
Length:18 bp
Location:5510 - 5528
M13 Reverse
Type:primer_bind
Length:17 bp
Location:362 - 379
M13/pUC Forward
Type:primer_bind
Length:23 bp
Location:5519 - 5542
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:343 - 366
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:7774 - 7794
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | → right | 363 - 380 | 17 bp | |
M13 fwd | primer_bind | ← left | 5511 - 5528 | 17 bp | |
U6 promoter | promoter | ← left | 5091 - 5340 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 4736 - 4961 | 225 bp | |
SV40 NLS | cds | → right | 4702 - 4723 | 21 bp | |
SV40 NLS | cds | → right | 862 - 883 | 21 bp | |
EF-1α core promoter | promoter | → right | 601 - 813 | 212 bp | |
f1 ori | origin | → right | 5669 - 6125 | 456 bp | |
ori | origin | → right | 7286 - 7875 | 589 bp | |
lac operator | protein_bind | → right | 339 - 356 | 17 bp | |
CAP binding site | protein_bind | → right | 265 - 287 | 22 bp | |
lac promoter | promoter | → right | 301 - 332 | 31 bp | |
AmpR | cds | → right | 6255 - 7116 | 861 bp | |
AmpR promoter | promoter | → right | 6150 - 6255 | 105 bp | |
AAV2 ITR | repeat_region | → right | 5363 - 5493 | 130 bp | |
AAV2 ITR | repeat_region | → right | 406 - 536 | 130 bp | |
Amp-R | primer_bind | ← left | 6472 - 6492 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 5965 - 5987 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 7626 - 7636 | 10 bp | |
F1ori-R | primer_bind | ← left | 5755 - 5775 | 20 bp | |
hU6-F (site 1) | primer_bind | → right | 52 - 62 | 10 bp | |
hU6-F (site 2) | primer_bind | ← left | 5318 - 5339 | 21 bp | |
LKO.1 5' | primer_bind | ← left | 5148 - 5168 | 20 bp | |
M13 Forward | primer_bind | ← left | 5510 - 5528 | 18 bp | |
M13 Reverse | primer_bind | → right | 362 - 379 | 17 bp | |
M13/pUC Forward | primer_bind | ← left | 5519 - 5542 | 23 bp | |
M13/pUC Reverse | primer_bind | → right | 343 - 366 | 23 bp | |
pBR322ori-F | primer_bind | → right | 7774 - 7794 | 20 bp |