Files
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A
Amir SaberiUpdate name and description
f71c237•1 month ago
pCMV-ABEmax-P2A-GFP
9627 bp
All features (34)
M13 rev
Type:primer_bind
Length:17 bp
Location:1247 - 1264
P2A
Type:cds
Length:57 bp
Location:91 - 148
6xHis
Type:cds
Length:18 bp
Location:906 - 924
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:952 - 1177
SV40 NLS
Type:cds
Length:21 bp
Location:4336 - 4357
SV40 NLS
Type:cds
Length:21 bp
Location:877 - 898
SV40 NLS
Type:cds
Length:21 bp
Location:61 - 82
CMV promoter
Type:promoter
Length:204 bp
Location:4023 - 4227
CMV enhancer
Type:enhancer
Length:380 bp
Location:3643 - 4023
EGFP
Type:cds
Length:717 bp
Location:148 - 865
T7 promoter
Type:promoter
Length:19 bp
Location:4268 - 4287
ori
Type:origin
Length:589 bp
Location:1649 - 2238
lac operator
Type:protein_bind
Length:17 bp
Location:1271 - 1288
CAP binding site
Type:protein_bind
Length:22 bp
Location:1340 - 1362
lac promoter
Type:promoter
Length:31 bp
Location:1295 - 1326
Cas9(D10A)
Type:cds
Length:27 bp
Location:5545 - 5572
AmpR
Type:cds
Length:861 bp
Location:2408 - 3269
AmpR promoter
Type:promoter
Length:105 bp
Location:3269 - 3374
Amp-R
Type:primer_bind
Length:20 bp
Location:3030 - 3050
BGH-rev (site 1)
Type:primer_bind
Length:18 bp
Location:945 - 963
BGH-rev (site 2)
Type:primer_bind
Length:16 bp
Location:5706 - 5722
CMV-F
Type:primer_bind
Length:21 bp
Location:4176 - 4197
EGFP-C (site 1)
Type:primer_bind
Length:22 bp
Location:800 - 822
EGFP-C (site 2)
Type:primer_bind
Length:10 bp
Location:8750 - 8760
EXFP-R (site 1)
Type:primer_bind
Length:20 bp
Location:453 - 473
EXFP-R (site 2)
Type:primer_bind
Length:10 bp
Location:6756 - 6766
L4440 (site 1)
Type:primer_bind
Length:18 bp
Location:1477 - 1495
L4440 (site 2)
Type:primer_bind
Length:10 bp
Location:3522 - 3532
L4440 (site 3)
Type:primer_bind
Length:10 bp
Location:8462 - 8472
M13 Reverse
Type:primer_bind
Length:17 bp
Location:1246 - 1263
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:1259 - 1282
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:1728 - 1748
pRS-marker
Type:primer_bind
Length:20 bp
Location:3451 - 3471
T7
Type:primer_bind
Length:20 bp
Location:4267 - 4287
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 1247 - 1264 | 17 bp | |
P2A | cds | → right | 91 - 148 | 57 bp | |
6xHis | cds | → right | 906 - 924 | 18 bp | |
bGH poly(A) signal | polya_signal | → right | 952 - 1177 | 225 bp | |
SV40 NLS | cds | → right | 4336 - 4357 | 21 bp | |
SV40 NLS | cds | → right | 877 - 898 | 21 bp | |
SV40 NLS | cds | → right | 61 - 82 | 21 bp | |
CMV promoter | promoter | → right | 4023 - 4227 | 204 bp | |
CMV enhancer | enhancer | → right | 3643 - 4023 | 380 bp | |
EGFP | cds | → right | 148 - 865 | 717 bp | |
T7 promoter | promoter | → right | 4268 - 4287 | 19 bp | |
ori | origin | ← left | 1649 - 2238 | 589 bp | |
lac operator | protein_bind | → right | 1271 - 1288 | 17 bp | |
CAP binding site | protein_bind | → right | 1340 - 1362 | 22 bp | |
lac promoter | promoter | ← left | 1295 - 1326 | 31 bp | |
Cas9(D10A) | cds | → right | 5545 - 5572 | 27 bp | |
AmpR | cds | ← left | 2408 - 3269 | 861 bp | |
AmpR promoter | promoter | ← left | 3269 - 3374 | 105 bp | |
Amp-R | primer_bind | → right | 3030 - 3050 | 20 bp | |
BGH-rev (site 1) | primer_bind | ← left | 945 - 963 | 18 bp | |
BGH-rev (site 2) | primer_bind | → right | 5706 - 5722 | 16 bp | |
CMV-F | primer_bind | → right | 4176 - 4197 | 21 bp | |
EGFP-C (site 1) | primer_bind | → right | 800 - 822 | 22 bp | |
EGFP-C (site 2) | primer_bind | → right | 8750 - 8760 | 10 bp | |
EXFP-R (site 1) | primer_bind | ← left | 453 - 473 | 20 bp | |
EXFP-R (site 2) | primer_bind | ← left | 6756 - 6766 | 10 bp | |
L4440 (site 1) | primer_bind | ← left | 1477 - 1495 | 18 bp | |
L4440 (site 2) | primer_bind | → right | 3522 - 3532 | 10 bp | |
L4440 (site 3) | primer_bind | → right | 8462 - 8472 | 10 bp | |
M13 Reverse | primer_bind | ← left | 1246 - 1263 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 1259 - 1282 | 23 bp | |
pBR322ori-F | primer_bind | ← left | 1728 - 1748 | 20 bp | |
pRS-marker | primer_bind | ← left | 3451 - 3471 | 20 bp | |
T7 | primer_bind | → right | 4267 - 4287 | 20 bp |