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Amir SaberiUpdate name and description
f71c237•1 month ago
pCMV-BE2
8532 bp
All features (27)
M13 rev
Type:primer_bind
Length:17 bp
Location:5887 - 5904
6xHis
Type:cds
Length:18 bp
Location:5546 - 5564
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:5592 - 5817
SV40 NLS
Type:cds
Length:21 bp
Location:5517 - 5538
CMV promoter
Type:promoter
Length:204 bp
Location:131 - 335
CMV enhancer
Type:enhancer
Length:-8152 bp
Location:8283 - 131
T7 promoter
Type:promoter
Length:19 bp
Location:376 - 395
ori
Type:origin
Length:589 bp
Location:6289 - 6878
lac operator
Type:protein_bind
Length:17 bp
Location:5911 - 5928
CAP binding site
Type:protein_bind
Length:22 bp
Location:5980 - 6002
lac promoter
Type:promoter
Length:31 bp
Location:5935 - 5966
UGI
Type:cds
Length:249 bp
Location:5256 - 5505
APOBEC-1
Type:cds
Length:687 bp
Location:408 - 1095
Cas9 VQR
Type:cds
Length:4101 bp
Location:1143 - 5244
dCas9
Type:cds
Length:4101 bp
Location:1143 - 5244
AmpR
Type:cds
Length:861 bp
Location:7048 - 7909
AmpR promoter
Type:promoter
Length:105 bp
Location:7909 - 8014
Amp-R
Type:primer_bind
Length:20 bp
Location:7670 - 7690
BGH-rev
Type:primer_bind
Length:18 bp
Location:5585 - 5603
CMV-F
Type:primer_bind
Length:21 bp
Location:284 - 305
L4440 (site 1)
Type:primer_bind
Length:18 bp
Location:6117 - 6135
L4440 (site 2)
Type:primer_bind
Length:10 bp
Location:8162 - 8172
M13 Reverse
Type:primer_bind
Length:17 bp
Location:5886 - 5903
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:5899 - 5922
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:6368 - 6388
pRS-marker
Type:primer_bind
Length:20 bp
Location:8091 - 8111
T7
Type:primer_bind
Length:20 bp
Location:375 - 395
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 5887 - 5904 | 17 bp | |
6xHis | cds | → right | 5546 - 5564 | 18 bp | |
bGH poly(A) signal | polya_signal | → right | 5592 - 5817 | 225 bp | |
SV40 NLS | cds | → right | 5517 - 5538 | 21 bp | |
CMV promoter | promoter | → right | 131 - 335 | 204 bp | |
CMV enhancer | enhancer | → right | 8283 - 131 | -8152 bp | |
T7 promoter | promoter | → right | 376 - 395 | 19 bp | |
ori | origin | ← left | 6289 - 6878 | 589 bp | |
lac operator | protein_bind | → right | 5911 - 5928 | 17 bp | |
CAP binding site | protein_bind | → right | 5980 - 6002 | 22 bp | |
lac promoter | promoter | ← left | 5935 - 5966 | 31 bp | |
UGI | cds | → right | 5256 - 5505 | 249 bp | |
APOBEC-1 | cds | → right | 408 - 1095 | 687 bp | |
Cas9 VQR | cds | → right | 1143 - 5244 | 4101 bp | |
dCas9 | cds | → right | 1143 - 5244 | 4101 bp | |
AmpR | cds | ← left | 7048 - 7909 | 861 bp | |
AmpR promoter | promoter | ← left | 7909 - 8014 | 105 bp | |
Amp-R | primer_bind | → right | 7670 - 7690 | 20 bp | |
BGH-rev | primer_bind | ← left | 5585 - 5603 | 18 bp | |
CMV-F | primer_bind | → right | 284 - 305 | 21 bp | |
L4440 (site 1) | primer_bind | ← left | 6117 - 6135 | 18 bp | |
L4440 (site 2) | primer_bind | → right | 8162 - 8172 | 10 bp | |
M13 Reverse | primer_bind | ← left | 5886 - 5903 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 5899 - 5922 | 23 bp | |
pBR322ori-F | primer_bind | ← left | 6368 - 6388 | 20 bp | |
pRS-marker | primer_bind | ← left | 8091 - 8111 | 20 bp | |
T7 | primer_bind | → right | 375 - 395 | 20 bp |