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A
Amir SaberiUpdate name and description
f71c237•1 month ago
pEJS-HZ79 All-in-one AAV-NmeABE8e
7471 bp
All features (18)
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:4846 - 4987
U6 promoter
Type:promoter
Length:249 bp
Location:4459 - 4708
SV40 NLS
Type:cds
Length:21 bp
Location:4375 - 4396
SV40 NLS
Type:cds
Length:21 bp
Location:442 - 463
f1 ori
Type:origin
Length:456 bp
Location:5061 - 5517
ori
Type:origin
Length:589 bp
Location:6821 - 7410
AmpR
Type:cds
Length:861 bp
Location:5790 - 6651
AmpR promoter
Type:promoter
Length:105 bp
Location:5685 - 5790
AAV2 ITR (alternate)
Type:repeat_region
Length:130 bp
Location:0 - 130
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:4846 - 4976
Amp-R
Type:primer_bind
Length:20 bp
Location:6007 - 6027
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:5357 - 5379
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:7161 - 7171
F1ori-R
Type:primer_bind
Length:20 bp
Location:5147 - 5167
hU6-F
Type:primer_bind
Length:21 bp
Location:4458 - 4479
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:4629 - 4649
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:7309 - 7329
pRS-marker
Type:primer_bind
Length:20 bp
Location:5532 - 5552
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
AAV2 ITR | repeat_region | → right | 4846 - 4987 | 141 bp | |
U6 promoter | promoter | → right | 4459 - 4708 | 249 bp | |
SV40 NLS | cds | → right | 4375 - 4396 | 21 bp | |
SV40 NLS | cds | → right | 442 - 463 | 21 bp | |
f1 ori | origin | → right | 5061 - 5517 | 456 bp | |
ori | origin | → right | 6821 - 7410 | 589 bp | |
AmpR | cds | → right | 5790 - 6651 | 861 bp | |
AmpR promoter | promoter | → right | 5685 - 5790 | 105 bp | |
AAV2 ITR (alternate) | repeat_region | → right | 0 - 130 | 130 bp | |
AAV2 ITR | repeat_region | → right | 4846 - 4976 | 130 bp | |
Amp-R | primer_bind | ← left | 6007 - 6027 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 5357 - 5379 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 7161 - 7171 | 10 bp | |
F1ori-R | primer_bind | ← left | 5147 - 5167 | 20 bp | |
hU6-F | primer_bind | → right | 4458 - 4479 | 21 bp | |
LKO.1 5' | primer_bind | → right | 4629 - 4649 | 20 bp | |
pBR322ori-F | primer_bind | → right | 7309 - 7329 | 20 bp | |
pRS-marker | primer_bind | ← left | 5532 - 5552 | 20 bp |