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Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
PX552
5292 bp
All features (27)
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:2554 - 2695
U6 promoter
Type:promoter
Length:249 bp
Location:144 - 393
hGH poly(A) signal
Type:polya_signal
Length:477 bp
Location:2038 - 2515
hSyn promoter
Type:promoter
Length:448 bp
Location:520 - 968
EGFP
Type:cds
Length:717 bp
Location:1005 - 1722
Kozak sequence
Type:regulatory
Length:10 bp
Location:999 - 1009
f1 ori
Type:origin
Length:456 bp
Location:2769 - 3225
ori
Type:origin
Length:589 bp
Location:4642 - 5231
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:411 - 487
AmpR
Type:cds
Length:861 bp
Location:3611 - 4472
AmpR promoter
Type:promoter
Length:105 bp
Location:3506 - 3611
AAV2 ITR (alternate)
Type:repeat_region
Length:130 bp
Location:0 - 130
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:2554 - 2684
Amp-R
Type:primer_bind
Length:20 bp
Location:3828 - 3848
EGFP-C
Type:primer_bind
Length:22 bp
Location:1657 - 1679
EGFP-N
Type:primer_bind
Length:22 bp
Location:1049 - 1071
EXFP-R
Type:primer_bind
Length:20 bp
Location:1310 - 1330
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:3065 - 3087
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:4982 - 4992
F1ori-R
Type:primer_bind
Length:20 bp
Location:2855 - 2875
hGH-PA-R
Type:primer_bind
Length:20 bp
Location:2236 - 2256
hU6-F
Type:primer_bind
Length:21 bp
Location:143 - 164
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:314 - 334
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:5130 - 5150
pBRforEco
Type:primer_bind
Length:19 bp
Location:3419 - 3438
pGEX 3'
Type:primer_bind
Length:23 bp
Location:3359 - 3382
pRS-marker
Type:primer_bind
Length:20 bp
Location:3240 - 3260
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
AAV2 ITR | repeat_region | → right | 2554 - 2695 | 141 bp | |
U6 promoter | promoter | → right | 144 - 393 | 249 bp | |
hGH poly(A) signal | polya_signal | → right | 2038 - 2515 | 477 bp | |
hSyn promoter | promoter | → right | 520 - 968 | 448 bp | |
EGFP | cds | → right | 1005 - 1722 | 717 bp | |
Kozak sequence | regulatory | → right | 999 - 1009 | 10 bp | |
f1 ori | origin | → right | 2769 - 3225 | 456 bp | |
ori | origin | → right | 4642 - 5231 | 589 bp | |
gRNA scaffold | misc_rna | → right | 411 - 487 | 76 bp | |
AmpR | cds | → right | 3611 - 4472 | 861 bp | |
AmpR promoter | promoter | → right | 3506 - 3611 | 105 bp | |
AAV2 ITR (alternate) | repeat_region | → right | 0 - 130 | 130 bp | |
AAV2 ITR | repeat_region | → right | 2554 - 2684 | 130 bp | |
Amp-R | primer_bind | ← left | 3828 - 3848 | 20 bp | |
EGFP-C | primer_bind | → right | 1657 - 1679 | 22 bp | |
EGFP-N | primer_bind | ← left | 1049 - 1071 | 22 bp | |
EXFP-R | primer_bind | ← left | 1310 - 1330 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 3065 - 3087 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 4982 - 4992 | 10 bp | |
F1ori-R | primer_bind | ← left | 2855 - 2875 | 20 bp | |
hGH-PA-R | primer_bind | ← left | 2236 - 2256 | 20 bp | |
hU6-F | primer_bind | → right | 143 - 164 | 21 bp | |
LKO.1 5' | primer_bind | → right | 314 - 334 | 20 bp | |
pBR322ori-F | primer_bind | → right | 5130 - 5150 | 20 bp | |
pBRforEco | primer_bind | ← left | 3419 - 3438 | 19 bp | |
pGEX 3' | primer_bind | → right | 3359 - 3382 | 23 bp | |
pRS-marker | primer_bind | ← left | 3240 - 3260 | 20 bp |