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A
Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
lentiCRISPR v2
14873 bp
All features (63)
M13 rev
Type:primer_bind
Length:17 bp
Location:12100 - 12117
Factor Xa site
Type:cds
Length:12 bp
Location:9817 - 9829
gp41 peptide
Type:cds
Length:45 bp
Location:1479 - 1524
P2A
Type:cds
Length:57 bp
Location:8677 - 8734
FLAG
Type:cds
Length:24 bp
Location:8644 - 8668
cPPT/CTS
Type:other
Length:118 bp
Location:1821 - 1939
RRE
Type:other
Length:234 bp
Location:1061 - 1295
HIV-1 Ψ
Type:other
Length:126 bp
Location:443 - 569
3' LTR (ΔU3)
Type:ltr
Length:234 bp
Location:10006 - 10240
5' LTR (truncated)
Type:ltr
Length:181 bp
Location:216 - 397
WPRE
Type:other
Length:589 bp
Location:9346 - 9935
U6 promoter
Type:promoter
Length:249 bp
Location:1989 - 2238
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:10271 - 10496
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:11930 - 12052
nucleoplasmin NLS
Type:cds
Length:48 bp
Location:8596 - 8644
SV40 ori
Type:origin
Length:136 bp
Location:11163 - 11299
SV40 promoter
Type:promoter
Length:330 bp
Location:10983 - 11313
EF-1α core promoter
Type:promoter
Length:212 bp
Location:4256 - 4468
CMV promoter
Type:promoter
Length:199 bp
Location:0 - 199
CMV enhancer
Type:enhancer
Length:380 bp
Location:14492 - 14872
EM7 promoter
Type:promoter
Length:48 bp
Location:11360 - 11408
Kozak sequence
Type:regulatory
Length:10 bp
Location:4486 - 4496
f1 ori
Type:origin
Length:429 bp
Location:10541 - 10970
ori
Type:origin
Length:589 bp
Location:12502 - 13091
lac operator
Type:protein_bind
Length:17 bp
Location:12124 - 12141
CAP binding site
Type:protein_bind
Length:22 bp
Location:12193 - 12215
lac promoter
Type:promoter
Length:31 bp
Location:12148 - 12179
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:4119 - 4195
Cas9
Type:cds
Length:4104 bp
Location:4492 - 8596
PuroR
Type:cds
Length:597 bp
Location:8734 - 9331
BleoR
Type:cds
Length:375 bp
Location:11426 - 11801
AmpR
Type:cds
Length:861 bp
Location:13261 - 14122
AmpR promoter
Type:promoter
Length:105 bp
Location:14122 - 14227
Amp-R
Type:primer_bind
Length:20 bp
Location:13883 - 13903
BGH-rev (site 1)
Type:primer_bind
Length:16 bp
Location:4656 - 4672
BGH-rev (site 2)
Type:primer_bind
Length:18 bp
Location:10264 - 10282
CMV-F
Type:primer_bind
Length:21 bp
Location:152 - 173
EBV-rev
Type:primer_bind
Length:20 bp
Location:12020 - 12040
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:7368 - 7378
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:10837 - 10859
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:12739 - 12749
F1ori-R
Type:primer_bind
Length:20 bp
Location:10627 - 10647
hU6-F (site 1)
Type:primer_bind
Length:21 bp
Location:1988 - 2009
hU6-F (site 2)
Type:primer_bind
Length:10 bp
Location:12416 - 12426
L4440 (site 1)
Type:primer_bind
Length:10 bp
Location:7412 - 7422
L4440 (site 2)
Type:primer_bind
Length:18 bp
Location:12330 - 12348
L4440 (site 3)
Type:primer_bind
Length:10 bp
Location:14371 - 14381
LKO.1 5' (site 1)
Type:primer_bind
Length:20 bp
Location:2159 - 2179
LKO.1 5' (site 2)
Type:primer_bind
Length:10 bp
Location:9455 - 9465
M13 Reverse
Type:primer_bind
Length:17 bp
Location:12099 - 12116
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:12112 - 12135
pBABE 3'
Type:primer_bind
Length:21 bp
Location:10977 - 10998
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:12581 - 12601
pRS-marker
Type:primer_bind
Length:20 bp
Location:14300 - 14320
Puro-F
Type:primer_bind
Length:21 bp
Location:9226 - 9247
SV40pA-R (site 1)
Type:primer_bind
Length:11 bp
Location:1198 - 1209
SV40pA-R (site 2)
Type:primer_bind
Length:10 bp
Location:9448 - 9458
SV40pA-R (site 3)
Type:primer_bind
Length:20 bp
Location:11966 - 11986
SV40pro-F (site 1)
Type:primer_bind
Length:20 bp
Location:11224 - 11244
SV40pro-F (site 2)
Type:primer_bind
Length:12 bp
Location:11247 - 11259
SV40pro-F (site 3)
Type:primer_bind
Length:10 bp
Location:11292 - 11302
WPRE-R (site 1)
Type:primer_bind
Length:12 bp
Location:1276 - 1288
WPRE-R (site 2)
Type:primer_bind
Length:21 bp
Location:9398 - 9419
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 12100 - 12117 | 17 bp | |
Factor Xa site | cds | ← left | 9817 - 9829 | 12 bp | |
gp41 peptide | cds | → right | 1479 - 1524 | 45 bp | |
P2A | cds | → right | 8677 - 8734 | 57 bp | |
FLAG | cds | → right | 8644 - 8668 | 24 bp | |
cPPT/CTS | other | → right | 1821 - 1939 | 118 bp | |
RRE | other | → right | 1061 - 1295 | 234 bp | |
HIV-1 Ψ | other | → right | 443 - 569 | 126 bp | |
3' LTR (ΔU3) | ltr | → right | 10006 - 10240 | 234 bp | |
5' LTR (truncated) | ltr | → right | 216 - 397 | 181 bp | |
WPRE | other | → right | 9346 - 9935 | 589 bp | |
U6 promoter | promoter | → right | 1989 - 2238 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 10271 - 10496 | 225 bp | |
SV40 poly(A) signal | polya_signal | → right | 11930 - 12052 | 122 bp | |
nucleoplasmin NLS | cds | → right | 8596 - 8644 | 48 bp | |
SV40 ori | origin | → right | 11163 - 11299 | 136 bp | |
SV40 promoter | promoter | → right | 10983 - 11313 | 330 bp | |
EF-1α core promoter | promoter | → right | 4256 - 4468 | 212 bp | |
CMV promoter | promoter | → right | 0 - 199 | 199 bp | |
CMV enhancer | enhancer | → right | 14492 - 14872 | 380 bp | |
EM7 promoter | promoter | → right | 11360 - 11408 | 48 bp | |
Kozak sequence | regulatory | → right | 4486 - 4496 | 10 bp | |
f1 ori | origin | → right | 10541 - 10970 | 429 bp | |
ori | origin | ← left | 12502 - 13091 | 589 bp | |
lac operator | protein_bind | → right | 12124 - 12141 | 17 bp | |
CAP binding site | protein_bind | → right | 12193 - 12215 | 22 bp | |
lac promoter | promoter | ← left | 12148 - 12179 | 31 bp | |
gRNA scaffold | misc_rna | → right | 4119 - 4195 | 76 bp | |
Cas9 | cds | → right | 4492 - 8596 | 4104 bp | |
PuroR | cds | → right | 8734 - 9331 | 597 bp | |
BleoR | cds | → right | 11426 - 11801 | 375 bp | |
AmpR | cds | ← left | 13261 - 14122 | 861 bp | |
AmpR promoter | promoter | ← left | 14122 - 14227 | 105 bp | |
Amp-R | primer_bind | → right | 13883 - 13903 | 20 bp | |
BGH-rev (site 1) | primer_bind | → right | 4656 - 4672 | 16 bp | |
BGH-rev (site 2) | primer_bind | ← left | 10264 - 10282 | 18 bp | |
CMV-F | primer_bind | → right | 152 - 173 | 21 bp | |
EBV-rev | primer_bind | → right | 12020 - 12040 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 7368 - 7378 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 10837 - 10859 | 22 bp | |
F1ori-F (site 3) | primer_bind | → right | 12739 - 12749 | 10 bp | |
F1ori-R | primer_bind | ← left | 10627 - 10647 | 20 bp | |
hU6-F (site 1) | primer_bind | → right | 1988 - 2009 | 21 bp | |
hU6-F (site 2) | primer_bind | ← left | 12416 - 12426 | 10 bp | |
L4440 (site 1) | primer_bind | → right | 7412 - 7422 | 10 bp | |
L4440 (site 2) | primer_bind | ← left | 12330 - 12348 | 18 bp | |
L4440 (site 3) | primer_bind | → right | 14371 - 14381 | 10 bp | |
LKO.1 5' (site 1) | primer_bind | → right | 2159 - 2179 | 20 bp | |
LKO.1 5' (site 2) | primer_bind | → right | 9455 - 9465 | 10 bp | |
M13 Reverse | primer_bind | ← left | 12099 - 12116 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 12112 - 12135 | 23 bp | |
pBABE 3' | primer_bind | ← left | 10977 - 10998 | 21 bp | |
pBR322ori-F | primer_bind | ← left | 12581 - 12601 | 20 bp | |
pRS-marker | primer_bind | ← left | 14300 - 14320 | 20 bp | |
Puro-F | primer_bind | → right | 9226 - 9247 | 21 bp | |
SV40pA-R (site 1) | primer_bind | ← left | 1198 - 1209 | 11 bp | |
SV40pA-R (site 2) | primer_bind | → right | 9448 - 9458 | 10 bp | |
SV40pA-R (site 3) | primer_bind | ← left | 11966 - 11986 | 20 bp | |
SV40pro-F (site 1) | primer_bind | → right | 11224 - 11244 | 20 bp | |
SV40pro-F (site 2) | primer_bind | ← left | 11247 - 11259 | 12 bp | |
SV40pro-F (site 3) | primer_bind | → right | 11292 - 11302 | 10 bp | |
WPRE-R (site 1) | primer_bind | → right | 1276 - 1288 | 12 bp | |
WPRE-R (site 2) | primer_bind | ← left | 9398 - 9419 | 21 bp |