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Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
pCAG-enAsCas12a(E174R/S542R/K548R)
8900 bp
All features (30)
M13 rev
Type:primer_bind
Length:17 bp
Location:6402 - 6419
3xHA
Type:cds
Length:81 bp
Location:5751 - 5832
β-globin poly(A) signal
Type:polya_signal
Length:56 bp
Location:5986 - 6042
SV40 poly(A) signal
Type:polya_signal
Length:135 bp
Location:6776 - 6911
chimeric intron
Type:intron
Length:1017 bp
Location:661 - 1678
nucleoplasmin NLS
Type:cds
Length:48 bp
Location:5697 - 5745
SV40 ori
Type:origin
Length:136 bp
Location:6621 - 6757
SV40 promoter
Type:promoter
Length:196 bp
Location:6575 - 6771
CMV enhancer
Type:enhancer
Length:380 bp
Location:4 - 384
Kozak sequence
Type:regulatory
Length:10 bp
Location:5416 - 5426
ori
Type:origin
Length:589 bp
Location:7149 - 7738
lac operator
Type:protein_bind
Length:17 bp
Location:6426 - 6443
CAP binding site
Type:protein_bind
Length:22 bp
Location:6495 - 6517
lac promoter
Type:promoter
Length:31 bp
Location:6450 - 6481
AsCpf1
Type:cds
Length:3921 bp
Location:1776 - 5697
AmpR
Type:cds
Length:861 bp
Location:7908 - 8769
AmpR promoter
Type:promoter
Length:105 bp
Location:8769 - 8874
Amp-R
Type:primer_bind
Length:20 bp
Location:8530 - 8550
Bglob-pA-R
Type:primer_bind
Length:20 bp
Location:5920 - 5940
EBV-rev
Type:primer_bind
Length:20 bp
Location:6866 - 6886
L4440 (site 1)
Type:primer_bind
Length:16 bp
Location:5254 - 5270
L4440 (site 2)
Type:primer_bind
Length:18 bp
Location:6977 - 6995
M13 Reverse (site 1)
Type:primer_bind
Length:11 bp
Location:6335 - 6346
M13 Reverse (site 2)
Type:primer_bind
Length:17 bp
Location:6401 - 6418
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:6414 - 6437
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:7228 - 7248
pCAG-F
Type:primer_bind
Length:20 bp
Location:1684 - 1704
pCAGGS-5
Type:primer_bind
Length:23 bp
Location:1600 - 1623
rbglobpA-R
Type:primer_bind
Length:20 bp
Location:6039 - 6059
SV40pA-R
Type:primer_bind
Length:20 bp
Location:6812 - 6832
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 6402 - 6419 | 17 bp | |
3xHA | cds | → right | 5751 - 5832 | 81 bp | |
β-globin poly(A) signal | polya_signal | → right | 5986 - 6042 | 56 bp | |
SV40 poly(A) signal | polya_signal | → right | 6776 - 6911 | 135 bp | |
chimeric intron | intron | → right | 661 - 1678 | 1017 bp | |
nucleoplasmin NLS | cds | → right | 5697 - 5745 | 48 bp | |
SV40 ori | origin | → right | 6621 - 6757 | 136 bp | |
SV40 promoter | promoter | → right | 6575 - 6771 | 196 bp | |
CMV enhancer | enhancer | → right | 4 - 384 | 380 bp | |
Kozak sequence | regulatory | → right | 5416 - 5426 | 10 bp | |
ori | origin | ← left | 7149 - 7738 | 589 bp | |
lac operator | protein_bind | → right | 6426 - 6443 | 17 bp | |
CAP binding site | protein_bind | → right | 6495 - 6517 | 22 bp | |
lac promoter | promoter | ← left | 6450 - 6481 | 31 bp | |
AsCpf1 | cds | → right | 1776 - 5697 | 3921 bp | |
AmpR | cds | ← left | 7908 - 8769 | 861 bp | |
AmpR promoter | promoter | ← left | 8769 - 8874 | 105 bp | |
Amp-R | primer_bind | → right | 8530 - 8550 | 20 bp | |
Bglob-pA-R | primer_bind | ← left | 5920 - 5940 | 20 bp | |
EBV-rev | primer_bind | → right | 6866 - 6886 | 20 bp | |
L4440 (site 1) | primer_bind | → right | 5254 - 5270 | 16 bp | |
L4440 (site 2) | primer_bind | ← left | 6977 - 6995 | 18 bp | |
M13 Reverse (site 1) | primer_bind | ← left | 6335 - 6346 | 11 bp | |
M13 Reverse (site 2) | primer_bind | ← left | 6401 - 6418 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 6414 - 6437 | 23 bp | |
pBR322ori-F | primer_bind | ← left | 7228 - 7248 | 20 bp | |
pCAG-F | primer_bind | → right | 1684 - 1704 | 20 bp | |
pCAGGS-5 | primer_bind | → right | 1600 - 1623 | 23 bp | |
rbglobpA-R | primer_bind | ← left | 6039 - 6059 | 20 bp | |
SV40pA-R | primer_bind | ← left | 6812 - 6832 | 20 bp |