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Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
pCMV-ABEmax
8811 bp
All features (28)
M13 rev
Type:primer_bind
Length:17 bp
Location:6166 - 6183
6xHis
Type:cds
Length:18 bp
Location:5825 - 5843
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:5871 - 6096
SV40 NLS
Type:cds
Length:21 bp
Location:5796 - 5817
SV40 NLS
Type:cds
Length:21 bp
Location:444 - 465
CMV promoter
Type:promoter
Length:204 bp
Location:131 - 335
CMV enhancer
Type:enhancer
Length:-8431 bp
Location:8562 - 131
T7 promoter
Type:promoter
Length:19 bp
Location:376 - 395
ori
Type:origin
Length:589 bp
Location:6568 - 7157
lac operator
Type:protein_bind
Length:17 bp
Location:6190 - 6207
CAP binding site
Type:protein_bind
Length:22 bp
Location:6259 - 6281
lac promoter
Type:promoter
Length:31 bp
Location:6214 - 6245
Cas9(D10A)
Type:cds
Length:4101 bp
Location:1653 - 5754
Cas9
Type:cds
Length:4101 bp
Location:1653 - 5754
AmpR
Type:cds
Length:861 bp
Location:7327 - 8188
AmpR promoter
Type:promoter
Length:105 bp
Location:8188 - 8293
Amp-R
Type:primer_bind
Length:20 bp
Location:7949 - 7969
BGH-rev (site 1)
Type:primer_bind
Length:16 bp
Location:1814 - 1830
BGH-rev (site 2)
Type:primer_bind
Length:18 bp
Location:5864 - 5882
CMV-F
Type:primer_bind
Length:21 bp
Location:284 - 305
L4440 (site 1)
Type:primer_bind
Length:10 bp
Location:4570 - 4580
L4440 (site 2)
Type:primer_bind
Length:18 bp
Location:6396 - 6414
L4440 (site 3)
Type:primer_bind
Length:10 bp
Location:8441 - 8451
M13 Reverse
Type:primer_bind
Length:17 bp
Location:6165 - 6182
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:6178 - 6201
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:6647 - 6667
pRS-marker
Type:primer_bind
Length:20 bp
Location:8370 - 8390
T7
Type:primer_bind
Length:20 bp
Location:375 - 395
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 6166 - 6183 | 17 bp | |
6xHis | cds | → right | 5825 - 5843 | 18 bp | |
bGH poly(A) signal | polya_signal | → right | 5871 - 6096 | 225 bp | |
SV40 NLS | cds | → right | 5796 - 5817 | 21 bp | |
SV40 NLS | cds | → right | 444 - 465 | 21 bp | |
CMV promoter | promoter | → right | 131 - 335 | 204 bp | |
CMV enhancer | enhancer | → right | 8562 - 131 | -8431 bp | |
T7 promoter | promoter | → right | 376 - 395 | 19 bp | |
ori | origin | ← left | 6568 - 7157 | 589 bp | |
lac operator | protein_bind | → right | 6190 - 6207 | 17 bp | |
CAP binding site | protein_bind | → right | 6259 - 6281 | 22 bp | |
lac promoter | promoter | ← left | 6214 - 6245 | 31 bp | |
Cas9(D10A) | cds | → right | 1653 - 5754 | 4101 bp | |
Cas9 | cds | → right | 1653 - 5754 | 4101 bp | |
AmpR | cds | ← left | 7327 - 8188 | 861 bp | |
AmpR promoter | promoter | ← left | 8188 - 8293 | 105 bp | |
Amp-R | primer_bind | → right | 7949 - 7969 | 20 bp | |
BGH-rev (site 1) | primer_bind | → right | 1814 - 1830 | 16 bp | |
BGH-rev (site 2) | primer_bind | ← left | 5864 - 5882 | 18 bp | |
CMV-F | primer_bind | → right | 284 - 305 | 21 bp | |
L4440 (site 1) | primer_bind | → right | 4570 - 4580 | 10 bp | |
L4440 (site 2) | primer_bind | ← left | 6396 - 6414 | 18 bp | |
L4440 (site 3) | primer_bind | → right | 8441 - 8451 | 10 bp | |
M13 Reverse | primer_bind | ← left | 6165 - 6182 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 6178 - 6201 | 23 bp | |
pBR322ori-F | primer_bind | ← left | 6647 - 6667 | 20 bp | |
pRS-marker | primer_bind | ← left | 8370 - 8390 | 20 bp | |
T7 | primer_bind | → right | 375 - 395 | 20 bp |