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A
Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
pLKO.1-puro-U6-sgRNA
7922 bp
All features (49)
M13 rev
Type:primer_bind
Length:17 bp
Location:6135 - 6152
M13 fwd
Type:primer_bind
Length:17 bp
Location:2609 - 2626
gp41 peptide
Type:cds
Length:45 bp
Location:7708 - 7753
IS1
Type:mobile_element
Length:768 bp
Location:5306 - 6074
cPPT/CTS
Type:other
Length:118 bp
Location:534 - 652
RRE
Type:other
Length:234 bp
Location:7290 - 7524
HIV-1 Ψ
Type:other
Length:126 bp
Location:6672 - 6798
3' LTR (ΔU3)
Type:ltr
Length:234 bp
Location:1959 - 2193
5' LTR (truncated)
Type:ltr
Length:181 bp
Location:6445 - 6626
hPGK promoter
Type:promoter
Length:511 bp
Location:700 - 1211
U6 promoter
Type:promoter
Length:249 bp
Location:128 - 377
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:2264 - 2386
SV40 ori
Type:origin
Length:136 bp
Location:2425 - 2561
RSV promoter
Type:promoter
Length:227 bp
Location:6218 - 6445
T3 promoter
Type:promoter
Length:19 bp
Location:6172 - 6191
T7 promoter
Type:promoter
Length:19 bp
Location:2581 - 2600
f1 ori
Type:origin
Length:456 bp
Location:2767 - 3223
ori
Type:origin
Length:589 bp
Location:4384 - 4973
lac operator
Type:protein_bind
Length:17 bp
Location:6111 - 6128
CAP binding site
Type:protein_bind
Length:22 bp
Location:5260 - 5282
lac promoter
Type:promoter
Length:30 bp
Location:6074 - 6104
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:410 - 486
PuroR
Type:cds
Length:600 bp
Location:1232 - 1832
AmpR
Type:cds
Length:861 bp
Location:3353 - 4214
AmpR promoter
Type:promoter
Length:105 bp
Location:3248 - 3353
Amp-R
Type:primer_bind
Length:20 bp
Location:3570 - 3590
EBV-rev
Type:primer_bind
Length:20 bp
Location:2354 - 2374
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:3063 - 3085
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:4724 - 4734
F1ori-R
Type:primer_bind
Length:20 bp
Location:2853 - 2873
hPGK-F
Type:primer_bind
Length:19 bp
Location:1128 - 1147
hU6-F (site 1)
Type:primer_bind
Length:21 bp
Location:127 - 148
hU6-F (site 2)
Type:primer_bind
Length:10 bp
Location:5047 - 5057
L4440
Type:primer_bind
Length:18 bp
Location:5125 - 5143
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:298 - 318
M13 Forward
Type:primer_bind
Length:18 bp
Location:2608 - 2626
M13 Reverse
Type:primer_bind
Length:17 bp
Location:6134 - 6151
M13/pUC Forward
Type:primer_bind
Length:23 bp
Location:2617 - 2640
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:6115 - 6138
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:4872 - 4892
Puro-F
Type:primer_bind
Length:21 bp
Location:1727 - 1748
Puro-R (site 1)
Type:primer_bind
Length:20 bp
Location:1231 - 1251
Puro-R (site 2)
Type:primer_bind
Length:10 bp
Location:1513 - 1523
SV40pA-R (site 1)
Type:primer_bind
Length:20 bp
Location:2300 - 2320
SV40pA-R (site 2)
Type:primer_bind
Length:11 bp
Location:7427 - 7438
T3 (site 1)
Type:primer_bind
Length:11 bp
Location:2580 - 2591
T3 (site 2)
Type:primer_bind
Length:21 bp
Location:6169 - 6190
T7 (site 1)
Type:primer_bind
Length:20 bp
Location:2579 - 2599
T7 (site 2)
Type:primer_bind
Length:12 bp
Location:6179 - 6191
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | → right | 6135 - 6152 | 17 bp | |
M13 fwd | primer_bind | ← left | 2609 - 2626 | 17 bp | |
gp41 peptide | cds | → right | 7708 - 7753 | 45 bp | |
IS1 | mobile_element | → right | 5306 - 6074 | 768 bp | |
cPPT/CTS | other | → right | 534 - 652 | 118 bp | |
RRE | other | → right | 7290 - 7524 | 234 bp | |
HIV-1 Ψ | other | → right | 6672 - 6798 | 126 bp | |
3' LTR (ΔU3) | ltr | → right | 1959 - 2193 | 234 bp | |
5' LTR (truncated) | ltr | → right | 6445 - 6626 | 181 bp | |
hPGK promoter | promoter | → right | 700 - 1211 | 511 bp | |
U6 promoter | promoter | → right | 128 - 377 | 249 bp | |
SV40 poly(A) signal | polya_signal | → right | 2264 - 2386 | 122 bp | |
SV40 ori | origin | → right | 2425 - 2561 | 136 bp | |
RSV promoter | promoter | → right | 6218 - 6445 | 227 bp | |
T3 promoter | promoter | → right | 6172 - 6191 | 19 bp | |
T7 promoter | promoter | ← left | 2581 - 2600 | 19 bp | |
f1 ori | origin | → right | 2767 - 3223 | 456 bp | |
ori | origin | → right | 4384 - 4973 | 589 bp | |
lac operator | protein_bind | → right | 6111 - 6128 | 17 bp | |
CAP binding site | protein_bind | → right | 5260 - 5282 | 22 bp | |
lac promoter | promoter | → right | 6074 - 6104 | 30 bp | |
gRNA scaffold | misc_rna | → right | 410 - 486 | 76 bp | |
PuroR | cds | → right | 1232 - 1832 | 600 bp | |
AmpR | cds | → right | 3353 - 4214 | 861 bp | |
AmpR promoter | promoter | → right | 3248 - 3353 | 105 bp | |
Amp-R | primer_bind | ← left | 3570 - 3590 | 20 bp | |
EBV-rev | primer_bind | → right | 2354 - 2374 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 3063 - 3085 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 4724 - 4734 | 10 bp | |
F1ori-R | primer_bind | ← left | 2853 - 2873 | 20 bp | |
hPGK-F | primer_bind | → right | 1128 - 1147 | 19 bp | |
hU6-F (site 1) | primer_bind | → right | 127 - 148 | 21 bp | |
hU6-F (site 2) | primer_bind | → right | 5047 - 5057 | 10 bp | |
L4440 | primer_bind | → right | 5125 - 5143 | 18 bp | |
LKO.1 5' | primer_bind | → right | 298 - 318 | 20 bp | |
M13 Forward | primer_bind | ← left | 2608 - 2626 | 18 bp | |
M13 Reverse | primer_bind | → right | 6134 - 6151 | 17 bp | |
M13/pUC Forward | primer_bind | ← left | 2617 - 2640 | 23 bp | |
M13/pUC Reverse | primer_bind | → right | 6115 - 6138 | 23 bp | |
pBR322ori-F | primer_bind | → right | 4872 - 4892 | 20 bp | |
Puro-F | primer_bind | → right | 1727 - 1748 | 21 bp | |
Puro-R (site 1) | primer_bind | ← left | 1231 - 1251 | 20 bp | |
Puro-R (site 2) | primer_bind | ← left | 1513 - 1523 | 10 bp | |
SV40pA-R (site 1) | primer_bind | ← left | 2300 - 2320 | 20 bp | |
SV40pA-R (site 2) | primer_bind | ← left | 7427 - 7438 | 11 bp | |
T3 (site 1) | primer_bind | ← left | 2580 - 2591 | 11 bp | |
T3 (site 2) | primer_bind | → right | 6169 - 6190 | 21 bp | |
T7 (site 1) | primer_bind | ← left | 2579 - 2599 | 20 bp | |
T7 (site 2) | primer_bind | → right | 6179 - 6191 | 12 bp |