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t
A
Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
pSpCas9(BB)-2A-Puro (PX459) V2.0
9171 bp
All features (34)
T2A
Type:cds
Length:54 bp
Location:5091 - 5145
3xFLAG
Type:cds
Length:66 bp
Location:810 - 876
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:5994 - 6135
U6 promoter
Type:promoter
Length:249 bp
Location:8732 - 8981
bGH poly(A) signal
Type:polya_signal
Length:208 bp
Location:5778 - 5986
hybrid intron
Type:intron
Length:228 bp
Location:562 - 790
SV40 NLS
Type:cds
Length:21 bp
Location:882 - 903
nucleoplasmin NLS
Type:cds
Length:48 bp
Location:5028 - 5076
CMV enhancer
Type:enhancer
Length:286 bp
Location:0 - 286
Kozak sequence
Type:regulatory
Length:10 bp
Location:801 - 811
f1 ori
Type:origin
Length:456 bp
Location:6209 - 6665
ori
Type:origin
Length:589 bp
Location:8082 - 8671
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:8999 - 9075
Cas9
Type:cds
Length:4101 bp
Location:927 - 5028
PuroR
Type:cds
Length:600 bp
Location:5145 - 5745
AmpR
Type:cds
Length:861 bp
Location:7051 - 7912
AmpR promoter
Type:promoter
Length:105 bp
Location:6946 - 7051
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:5994 - 6124
Amp-R
Type:primer_bind
Length:20 bp
Location:7268 - 7288
BGH-rev (site 1)
Type:primer_bind
Length:16 bp
Location:1088 - 1104
BGH-rev (site 2)
Type:primer_bind
Length:18 bp
Location:5771 - 5789
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:3800 - 3810
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:6505 - 6527
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:8422 - 8432
F1ori-R
Type:primer_bind
Length:20 bp
Location:6295 - 6315
hU6-F
Type:primer_bind
Length:21 bp
Location:8731 - 8752
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:8902 - 8922
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:8570 - 8590
pBRforEco
Type:primer_bind
Length:19 bp
Location:6859 - 6878
pGEX 3'
Type:primer_bind
Length:23 bp
Location:6799 - 6822
pRS-marker
Type:primer_bind
Length:20 bp
Location:6680 - 6700
Puro-F
Type:primer_bind
Length:21 bp
Location:5640 - 5661
Puro-R (site 1)
Type:primer_bind
Length:20 bp
Location:5144 - 5164
Puro-R (site 2)
Type:primer_bind
Length:10 bp
Location:5426 - 5436
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
T2A | cds | → right | 5091 - 5145 | 54 bp | |
3xFLAG | cds | → right | 810 - 876 | 66 bp | |
AAV2 ITR | repeat_region | → right | 5994 - 6135 | 141 bp | |
U6 promoter | promoter | → right | 8732 - 8981 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 5778 - 5986 | 208 bp | |
hybrid intron | intron | → right | 562 - 790 | 228 bp | |
SV40 NLS | cds | → right | 882 - 903 | 21 bp | |
nucleoplasmin NLS | cds | → right | 5028 - 5076 | 48 bp | |
CMV enhancer | enhancer | → right | 0 - 286 | 286 bp | |
Kozak sequence | regulatory | → right | 801 - 811 | 10 bp | |
f1 ori | origin | → right | 6209 - 6665 | 456 bp | |
ori | origin | → right | 8082 - 8671 | 589 bp | |
gRNA scaffold | misc_rna | → right | 8999 - 9075 | 76 bp | |
Cas9 | cds | → right | 927 - 5028 | 4101 bp | |
PuroR | cds | → right | 5145 - 5745 | 600 bp | |
AmpR | cds | → right | 7051 - 7912 | 861 bp | |
AmpR promoter | promoter | → right | 6946 - 7051 | 105 bp | |
AAV2 ITR | repeat_region | → right | 5994 - 6124 | 130 bp | |
Amp-R | primer_bind | ← left | 7268 - 7288 | 20 bp | |
BGH-rev (site 1) | primer_bind | → right | 1088 - 1104 | 16 bp | |
BGH-rev (site 2) | primer_bind | ← left | 5771 - 5789 | 18 bp | |
F1ori-F (site 1) | primer_bind | → right | 3800 - 3810 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 6505 - 6527 | 22 bp | |
F1ori-F (site 3) | primer_bind | ← left | 8422 - 8432 | 10 bp | |
F1ori-R | primer_bind | ← left | 6295 - 6315 | 20 bp | |
hU6-F | primer_bind | → right | 8731 - 8752 | 21 bp | |
LKO.1 5' | primer_bind | → right | 8902 - 8922 | 20 bp | |
pBR322ori-F | primer_bind | → right | 8570 - 8590 | 20 bp | |
pBRforEco | primer_bind | ← left | 6859 - 6878 | 19 bp | |
pGEX 3' | primer_bind | → right | 6799 - 6822 | 23 bp | |
pRS-marker | primer_bind | ← left | 6680 - 6700 | 20 bp | |
Puro-F | primer_bind | → right | 5640 - 5661 | 21 bp | |
Puro-R (site 1) | primer_bind | ← left | 5144 - 5164 | 20 bp | |
Puro-R (site 2) | primer_bind | ← left | 5426 - 5436 | 10 bp |