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t
A
Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
pSpCas9n(BB)-2A-Puro (PX462) V2.0
9176 bp
All features (36)
T2A
Type:cds
Length:54 bp
Location:5535 - 5589
3xFLAG
Type:cds
Length:66 bp
Location:1254 - 1320
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:6438 - 6579
U6 promoter
Type:promoter
Length:249 bp
Location:0 - 249
bGH poly(A) signal
Type:polya_signal
Length:208 bp
Location:6222 - 6430
hybrid intron
Type:intron
Length:228 bp
Location:1006 - 1234
SV40 NLS
Type:cds
Length:21 bp
Location:1326 - 1347
nucleoplasmin NLS
Type:cds
Length:48 bp
Location:5472 - 5520
chicken β-actin promoter
Type:promoter
Length:280 bp
Location:726 - 1006
CMV enhancer
Type:enhancer
Length:286 bp
Location:439 - 725
Kozak sequence
Type:regulatory
Length:10 bp
Location:1245 - 1255
f1 ori
Type:origin
Length:456 bp
Location:6653 - 7109
ori
Type:origin
Length:589 bp
Location:8526 - 9115
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:267 - 343
Cas9(D10A)
Type:cds
Length:4101 bp
Location:1371 - 5472
Cas9
Type:cds
Length:4101 bp
Location:1371 - 5472
PuroR
Type:cds
Length:600 bp
Location:5589 - 6189
AmpR
Type:cds
Length:861 bp
Location:7495 - 8356
AmpR promoter
Type:promoter
Length:105 bp
Location:7390 - 7495
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:6438 - 6568
Amp-R
Type:primer_bind
Length:20 bp
Location:7712 - 7732
BGH-rev (site 1)
Type:primer_bind
Length:16 bp
Location:1532 - 1548
BGH-rev (site 2)
Type:primer_bind
Length:18 bp
Location:6215 - 6233
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:4244 - 4254
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:6949 - 6971
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:8866 - 8876
F1ori-R
Type:primer_bind
Length:20 bp
Location:6739 - 6759
hU6-F
Type:primer_bind
Length:21 bp
Location:-1 - 20
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:170 - 190
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:9014 - 9034
pBRforEco
Type:primer_bind
Length:19 bp
Location:7303 - 7322
pGEX 3'
Type:primer_bind
Length:23 bp
Location:7243 - 7266
pRS-marker
Type:primer_bind
Length:20 bp
Location:7124 - 7144
Puro-F
Type:primer_bind
Length:21 bp
Location:6084 - 6105
Puro-R (site 1)
Type:primer_bind
Length:20 bp
Location:5588 - 5608
Puro-R (site 2)
Type:primer_bind
Length:10 bp
Location:5870 - 5880
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
T2A | cds | → right | 5535 - 5589 | 54 bp | |
3xFLAG | cds | → right | 1254 - 1320 | 66 bp | |
AAV2 ITR | repeat_region | → right | 6438 - 6579 | 141 bp | |
U6 promoter | promoter | → right | 0 - 249 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 6222 - 6430 | 208 bp | |
hybrid intron | intron | → right | 1006 - 1234 | 228 bp | |
SV40 NLS | cds | → right | 1326 - 1347 | 21 bp | |
nucleoplasmin NLS | cds | → right | 5472 - 5520 | 48 bp | |
chicken β-actin promoter | promoter | → right | 726 - 1006 | 280 bp | |
CMV enhancer | enhancer | → right | 439 - 725 | 286 bp | |
Kozak sequence | regulatory | → right | 1245 - 1255 | 10 bp | |
f1 ori | origin | → right | 6653 - 7109 | 456 bp | |
ori | origin | → right | 8526 - 9115 | 589 bp | |
gRNA scaffold | misc_rna | → right | 267 - 343 | 76 bp | |
Cas9(D10A) | cds | → right | 1371 - 5472 | 4101 bp | |
Cas9 | cds | → right | 1371 - 5472 | 4101 bp | |
PuroR | cds | → right | 5589 - 6189 | 600 bp | |
AmpR | cds | → right | 7495 - 8356 | 861 bp | |
AmpR promoter | promoter | → right | 7390 - 7495 | 105 bp | |
AAV2 ITR | repeat_region | → right | 6438 - 6568 | 130 bp | |
Amp-R | primer_bind | ← left | 7712 - 7732 | 20 bp | |
BGH-rev (site 1) | primer_bind | → right | 1532 - 1548 | 16 bp | |
BGH-rev (site 2) | primer_bind | ← left | 6215 - 6233 | 18 bp | |
F1ori-F (site 1) | primer_bind | → right | 4244 - 4254 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 6949 - 6971 | 22 bp | |
F1ori-F (site 3) | primer_bind | ← left | 8866 - 8876 | 10 bp | |
F1ori-R | primer_bind | ← left | 6739 - 6759 | 20 bp | |
hU6-F | primer_bind | → right | -1 - 20 | 21 bp | |
LKO.1 5' | primer_bind | → right | 170 - 190 | 20 bp | |
pBR322ori-F | primer_bind | → right | 9014 - 9034 | 20 bp | |
pBRforEco | primer_bind | ← left | 7303 - 7322 | 19 bp | |
pGEX 3' | primer_bind | → right | 7243 - 7266 | 23 bp | |
pRS-marker | primer_bind | ← left | 7124 - 7144 | 20 bp | |
Puro-F | primer_bind | → right | 6084 - 6105 | 21 bp | |
Puro-R (site 1) | primer_bind | ← left | 5588 - 5608 | 20 bp | |
Puro-R (site 2) | primer_bind | ← left | 5870 - 5880 | 10 bp |