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Amir SaberiUpdate names and metadata
dbe1b11•1 month ago
pX330-U6-Chimeric_BB-CBh-hSpCas9
8504 bp
All features (30)
3xFLAG
Type:cds
Length:66 bp
Location:1256 - 1322
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:5771 - 5912
U6 promoter
Type:promoter
Length:249 bp
Location:5 - 254
bGH poly(A) signal
Type:polya_signal
Length:208 bp
Location:5555 - 5763
hybrid intron
Type:intron
Length:228 bp
Location:1008 - 1236
SV40 NLS
Type:cds
Length:21 bp
Location:1328 - 1349
nucleoplasmin NLS
Type:cds
Length:48 bp
Location:5474 - 5522
chicken β-actin promoter
Type:promoter
Length:277 bp
Location:731 - 1008
CMV enhancer
Type:enhancer
Length:286 bp
Location:444 - 730
Kozak sequence
Type:regulatory
Length:10 bp
Location:1247 - 1257
f1 ori
Type:origin
Length:456 bp
Location:5986 - 6442
ori
Type:origin
Length:589 bp
Location:7859 - 8448
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:272 - 348
Cas9
Type:cds
Length:4101 bp
Location:1373 - 5474
AmpR
Type:cds
Length:861 bp
Location:6828 - 7689
AmpR promoter
Type:promoter
Length:105 bp
Location:6723 - 6828
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:5771 - 5901
Amp-R
Type:primer_bind
Length:20 bp
Location:7045 - 7065
BGH-rev (site 1)
Type:primer_bind
Length:16 bp
Location:1534 - 1550
BGH-rev (site 2)
Type:primer_bind
Length:18 bp
Location:5548 - 5566
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:4246 - 4256
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:6282 - 6304
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:8199 - 8209
F1ori-R
Type:primer_bind
Length:20 bp
Location:6072 - 6092
hU6-F
Type:primer_bind
Length:21 bp
Location:4 - 25
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:175 - 195
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:8347 - 8367
pBRforEco
Type:primer_bind
Length:19 bp
Location:6636 - 6655
pGEX 3'
Type:primer_bind
Length:23 bp
Location:6576 - 6599
pRS-marker
Type:primer_bind
Length:20 bp
Location:6457 - 6477
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
3xFLAG | cds | → right | 1256 - 1322 | 66 bp | |
AAV2 ITR | repeat_region | → right | 5771 - 5912 | 141 bp | |
U6 promoter | promoter | → right | 5 - 254 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 5555 - 5763 | 208 bp | |
hybrid intron | intron | → right | 1008 - 1236 | 228 bp | |
SV40 NLS | cds | → right | 1328 - 1349 | 21 bp | |
nucleoplasmin NLS | cds | → right | 5474 - 5522 | 48 bp | |
chicken β-actin promoter | promoter | → right | 731 - 1008 | 277 bp | |
CMV enhancer | enhancer | → right | 444 - 730 | 286 bp | |
Kozak sequence | regulatory | → right | 1247 - 1257 | 10 bp | |
f1 ori | origin | → right | 5986 - 6442 | 456 bp | |
ori | origin | → right | 7859 - 8448 | 589 bp | |
gRNA scaffold | misc_rna | → right | 272 - 348 | 76 bp | |
Cas9 | cds | → right | 1373 - 5474 | 4101 bp | |
AmpR | cds | → right | 6828 - 7689 | 861 bp | |
AmpR promoter | promoter | → right | 6723 - 6828 | 105 bp | |
AAV2 ITR | repeat_region | → right | 5771 - 5901 | 130 bp | |
Amp-R | primer_bind | ← left | 7045 - 7065 | 20 bp | |
BGH-rev (site 1) | primer_bind | → right | 1534 - 1550 | 16 bp | |
BGH-rev (site 2) | primer_bind | ← left | 5548 - 5566 | 18 bp | |
F1ori-F (site 1) | primer_bind | → right | 4246 - 4256 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 6282 - 6304 | 22 bp | |
F1ori-F (site 3) | primer_bind | ← left | 8199 - 8209 | 10 bp | |
F1ori-R | primer_bind | ← left | 6072 - 6092 | 20 bp | |
hU6-F | primer_bind | → right | 4 - 25 | 21 bp | |
LKO.1 5' | primer_bind | → right | 175 - 195 | 20 bp | |
pBR322ori-F | primer_bind | → right | 8347 - 8367 | 20 bp | |
pBRforEco | primer_bind | ← left | 6636 - 6655 | 19 bp | |
pGEX 3' | primer_bind | → right | 6576 - 6599 | 23 bp | |
pRS-marker | primer_bind | ← left | 6457 - 6477 | 20 bp |