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Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
Luciferase-pcDNA3
7040 bp
All features (42)
M13 rev
Type:primer_bind
Length:17 bp
Location:1643 - 1660
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:6437 - 6662
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:1473 - 1595
SV40 ori
Type:origin
Length:136 bp
Location:289 - 425
SV40 promoter
Type:promoter
Length:330 bp
Location:109 - 439
CMV promoter
Type:promoter
Length:204 bp
Location:4415 - 4619
CMV enhancer
Type:enhancer
Length:380 bp
Location:4035 - 4415
luciferase
Type:cds
Length:1653 bp
Location:4724 - 6377
SP6 promoter
Type:promoter
Length:19 bp
Location:6393 - 6412
T7 promoter
Type:promoter
Length:19 bp
Location:4663 - 4682
Kozak sequence
Type:regulatory
Length:10 bp
Location:4718 - 4728
f1 ori
Type:origin
Length:-6611 bp
Location:6707 - 96
ori
Type:origin
Length:589 bp
Location:2045 - 2634
lac operator
Type:protein_bind
Length:17 bp
Location:1667 - 1684
CAP binding site
Type:protein_bind
Length:22 bp
Location:1736 - 1758
lac promoter
Type:promoter
Length:31 bp
Location:1691 - 1722
NeoR/KanR
Type:cds
Length:795 bp
Location:505 - 1300
AmpR
Type:cds
Length:861 bp
Location:2804 - 3665
AmpR promoter
Type:promoter
Length:105 bp
Location:3665 - 3770
Amp-R
Type:primer_bind
Length:20 bp
Location:3426 - 3446
BGH-rev
Type:primer_bind
Length:18 bp
Location:6430 - 6448
CMV-F
Type:primer_bind
Length:21 bp
Location:4568 - 4589
EBV-rev
Type:primer_bind
Length:20 bp
Location:1563 - 1583
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:2282 - 2292
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:7003 - 7025
F1ori-R
Type:primer_bind
Length:20 bp
Location:6793 - 6813
L4440 (site 1)
Type:primer_bind
Length:18 bp
Location:1873 - 1891
L4440 (site 2)
Type:primer_bind
Length:10 bp
Location:3914 - 3924
LucNrev
Type:primer_bind
Length:19 bp
Location:4789 - 4808
M13 Reverse
Type:primer_bind
Length:17 bp
Location:1642 - 1659
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:1655 - 1678
Neo-F
Type:primer_bind
Length:20 bp
Location:1168 - 1188
Neo-R
Type:primer_bind
Length:20 bp
Location:558 - 578
pBABE 3'
Type:primer_bind
Length:21 bp
Location:103 - 124
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:2124 - 2144
pRS-marker
Type:primer_bind
Length:20 bp
Location:3843 - 3863
SP6
Type:primer_bind
Length:18 bp
Location:6393 - 6411
SV40pA-R
Type:primer_bind
Length:20 bp
Location:1509 - 1529
SV40pro-F (site 1)
Type:primer_bind
Length:20 bp
Location:350 - 370
SV40pro-F (site 2)
Type:primer_bind
Length:12 bp
Location:373 - 385
SV40pro-F (site 3)
Type:primer_bind
Length:10 bp
Location:418 - 428
T7
Type:primer_bind
Length:20 bp
Location:4662 - 4682
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 1643 - 1660 | 17 bp | |
bGH poly(A) signal | polya_signal | → right | 6437 - 6662 | 225 bp | |
SV40 poly(A) signal | polya_signal | → right | 1473 - 1595 | 122 bp | |
SV40 ori | origin | → right | 289 - 425 | 136 bp | |
SV40 promoter | promoter | → right | 109 - 439 | 330 bp | |
CMV promoter | promoter | → right | 4415 - 4619 | 204 bp | |
CMV enhancer | enhancer | → right | 4035 - 4415 | 380 bp | |
luciferase | cds | → right | 4724 - 6377 | 1653 bp | |
SP6 promoter | promoter | ← left | 6393 - 6412 | 19 bp | |
T7 promoter | promoter | → right | 4663 - 4682 | 19 bp | |
Kozak sequence | regulatory | → right | 4718 - 4728 | 10 bp | |
f1 ori | origin | → right | 6707 - 96 | -6611 bp | |
ori | origin | ← left | 2045 - 2634 | 589 bp | |
lac operator | protein_bind | → right | 1667 - 1684 | 17 bp | |
CAP binding site | protein_bind | → right | 1736 - 1758 | 22 bp | |
lac promoter | promoter | ← left | 1691 - 1722 | 31 bp | |
NeoR/KanR | cds | → right | 505 - 1300 | 795 bp | |
AmpR | cds | ← left | 2804 - 3665 | 861 bp | |
AmpR promoter | promoter | ← left | 3665 - 3770 | 105 bp | |
Amp-R | primer_bind | → right | 3426 - 3446 | 20 bp | |
BGH-rev | primer_bind | ← left | 6430 - 6448 | 18 bp | |
CMV-F | primer_bind | → right | 4568 - 4589 | 21 bp | |
EBV-rev | primer_bind | → right | 1563 - 1583 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 2282 - 2292 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 7003 - 7025 | 22 bp | |
F1ori-R | primer_bind | ← left | 6793 - 6813 | 20 bp | |
L4440 (site 1) | primer_bind | ← left | 1873 - 1891 | 18 bp | |
L4440 (site 2) | primer_bind | → right | 3914 - 3924 | 10 bp | |
LucNrev | primer_bind | ← left | 4789 - 4808 | 19 bp | |
M13 Reverse | primer_bind | ← left | 1642 - 1659 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 1655 - 1678 | 23 bp | |
Neo-F | primer_bind | → right | 1168 - 1188 | 20 bp | |
Neo-R | primer_bind | ← left | 558 - 578 | 20 bp | |
pBABE 3' | primer_bind | ← left | 103 - 124 | 21 bp | |
pBR322ori-F | primer_bind | ← left | 2124 - 2144 | 20 bp | |
pRS-marker | primer_bind | ← left | 3843 - 3863 | 20 bp | |
SP6 | primer_bind | ← left | 6393 - 6411 | 18 bp | |
SV40pA-R | primer_bind | ← left | 1509 - 1529 | 20 bp | |
SV40pro-F (site 1) | primer_bind | → right | 350 - 370 | 20 bp | |
SV40pro-F (site 2) | primer_bind | ← left | 373 - 385 | 12 bp | |
SV40pro-F (site 3) | primer_bind | → right | 418 - 428 | 10 bp | |
T7 | primer_bind | → right | 4662 - 4682 | 20 bp |