Files
t
A
Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pAAV-CAG-GFP
5439 bp
All features (27)
Factor Xa site
Type:cds
Length:12 bp
Location:4811 - 4823
WPRE
Type:other
Length:589 bp
Location:4340 - 4929
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:4971 - 5093
chicken β-actin promoter
Type:promoter
Length:278 bp
Location:2898 - 3176
EGFP
Type:cds
Length:717 bp
Location:3596 - 4313
EGFP
Type:cds
Length:720 bp
Location:3596 - 4316
Kozak sequence
Type:regulatory
Length:10 bp
Location:3590 - 3600
f1 ori
Type:origin
Length:456 bp
Location:1931 - 2387
ori
Type:origin
Length:589 bp
Location:181 - 770
AmpR
Type:cds
Length:861 bp
Location:940 - 1801
AmpR promoter
Type:promoter
Length:105 bp
Location:1801 - 1906
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:5254 - 5384
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:2458 - 2588
Amp-R
Type:primer_bind
Length:20 bp
Location:1562 - 1582
EBV-rev
Type:primer_bind
Length:20 bp
Location:4981 - 5001
EGFP-C
Type:primer_bind
Length:22 bp
Location:4248 - 4270
EGFP-N
Type:primer_bind
Length:22 bp
Location:3640 - 3662
EXFP-R
Type:primer_bind
Length:20 bp
Location:3901 - 3921
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:418 - 428
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:2067 - 2089
F1ori-R
Type:primer_bind
Length:20 bp
Location:2279 - 2299
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:260 - 280
pCAG-F
Type:primer_bind
Length:20 bp
Location:3537 - 3557
pCAGGS-5
Type:primer_bind
Length:23 bp
Location:3453 - 3476
SV40pA-R (site 1)
Type:primer_bind
Length:10 bp
Location:4442 - 4452
SV40pA-R (site 2)
Type:primer_bind
Length:20 bp
Location:5035 - 5055
WPRE-R
Type:primer_bind
Length:21 bp
Location:4392 - 4413
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
Factor Xa site | cds | ← left | 4811 - 4823 | 12 bp | |
WPRE | other | → right | 4340 - 4929 | 589 bp | |
SV40 poly(A) signal | polya_signal | → right | 4971 - 5093 | 122 bp | |
chicken β-actin promoter | promoter | → right | 2898 - 3176 | 278 bp | |
EGFP | cds | → right | 3596 - 4313 | 717 bp | |
EGFP | cds | → right | 3596 - 4316 | 720 bp | |
Kozak sequence | regulatory | → right | 3590 - 3600 | 10 bp | |
f1 ori | origin | ← left | 1931 - 2387 | 456 bp | |
ori | origin | ← left | 181 - 770 | 589 bp | |
AmpR | cds | ← left | 940 - 1801 | 861 bp | |
AmpR promoter | promoter | ← left | 1801 - 1906 | 105 bp | |
AAV2 ITR | repeat_region | → right | 5254 - 5384 | 130 bp | |
AAV2 ITR | repeat_region | → right | 2458 - 2588 | 130 bp | |
Amp-R | primer_bind | → right | 1562 - 1582 | 20 bp | |
EBV-rev | primer_bind | ← left | 4981 - 5001 | 20 bp | |
EGFP-C | primer_bind | → right | 4248 - 4270 | 22 bp | |
EGFP-N | primer_bind | ← left | 3640 - 3662 | 22 bp | |
EXFP-R | primer_bind | ← left | 3901 - 3921 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 418 - 428 | 10 bp | |
F1ori-F (site 2) | primer_bind | ← left | 2067 - 2089 | 22 bp | |
F1ori-R | primer_bind | → right | 2279 - 2299 | 20 bp | |
pBR322ori-F | primer_bind | ← left | 260 - 280 | 20 bp | |
pCAG-F | primer_bind | → right | 3537 - 3557 | 20 bp | |
pCAGGS-5 | primer_bind | → right | 3453 - 3476 | 23 bp | |
SV40pA-R (site 1) | primer_bind | → right | 4442 - 4452 | 10 bp | |
SV40pA-R (site 2) | primer_bind | → right | 5035 - 5055 | 20 bp | |
WPRE-R | primer_bind | ← left | 4392 - 4413 | 21 bp |