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Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pAAV-CAG-tdTomato (codon diversified)
6161 bp
All features (23)
Factor Xa site
Type:cds
Length:12 bp
Location:3075 - 3087
WPRE
Type:other
Length:589 bp
Location:2604 - 3193
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:3235 - 3357
chicken β-actin promoter
Type:promoter
Length:283 bp
Location:440 - 723
Kozak sequence
Type:regulatory
Length:10 bp
Location:1143 - 1153
f1 ori
Type:origin
Length:456 bp
Location:5634 - 6090
ori
Type:origin
Length:589 bp
Location:3884 - 4473
AmpR
Type:cds
Length:861 bp
Location:4643 - 5504
AmpR promoter
Type:promoter
Length:105 bp
Location:5504 - 5609
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:3518 - 3648
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:0 - 130
Amp-R
Type:primer_bind
Length:20 bp
Location:5265 - 5285
EBV-rev
Type:primer_bind
Length:20 bp
Location:3245 - 3265
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:4121 - 4131
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:5770 - 5792
F1ori-R (site 1)
Type:primer_bind
Length:10 bp
Location:1974 - 1984
F1ori-R (site 2)
Type:primer_bind
Length:20 bp
Location:5982 - 6002
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:3963 - 3983
pCAG-F
Type:primer_bind
Length:20 bp
Location:1084 - 1104
pCAGGS-5
Type:primer_bind
Length:23 bp
Location:1000 - 1023
SV40pA-R (site 1)
Type:primer_bind
Length:10 bp
Location:2706 - 2716
SV40pA-R (site 2)
Type:primer_bind
Length:20 bp
Location:3299 - 3319
WPRE-R
Type:primer_bind
Length:21 bp
Location:2656 - 2677
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
Factor Xa site | cds | ← left | 3075 - 3087 | 12 bp | |
WPRE | other | → right | 2604 - 3193 | 589 bp | |
SV40 poly(A) signal | polya_signal | → right | 3235 - 3357 | 122 bp | |
chicken β-actin promoter | promoter | → right | 440 - 723 | 283 bp | |
Kozak sequence | regulatory | → right | 1143 - 1153 | 10 bp | |
f1 ori | origin | ← left | 5634 - 6090 | 456 bp | |
ori | origin | ← left | 3884 - 4473 | 589 bp | |
AmpR | cds | ← left | 4643 - 5504 | 861 bp | |
AmpR promoter | promoter | ← left | 5504 - 5609 | 105 bp | |
AAV2 ITR | repeat_region | → right | 3518 - 3648 | 130 bp | |
AAV2 ITR | repeat_region | → right | 0 - 130 | 130 bp | |
Amp-R | primer_bind | → right | 5265 - 5285 | 20 bp | |
EBV-rev | primer_bind | ← left | 3245 - 3265 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 4121 - 4131 | 10 bp | |
F1ori-F (site 2) | primer_bind | ← left | 5770 - 5792 | 22 bp | |
F1ori-R (site 1) | primer_bind | → right | 1974 - 1984 | 10 bp | |
F1ori-R (site 2) | primer_bind | → right | 5982 - 6002 | 20 bp | |
pBR322ori-F | primer_bind | ← left | 3963 - 3983 | 20 bp | |
pCAG-F | primer_bind | → right | 1084 - 1104 | 20 bp | |
pCAGGS-5 | primer_bind | → right | 1000 - 1023 | 23 bp | |
SV40pA-R (site 1) | primer_bind | → right | 2706 - 2716 | 10 bp | |
SV40pA-R (site 2) | primer_bind | → right | 3299 - 3319 | 20 bp | |
WPRE-R | primer_bind | ← left | 2656 - 2677 | 21 bp |