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Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pAAV-hSyn-DIO-mCherry
5524 bp
All features (29)
lox2272
Type:protein_bind
Length:34 bp
Location:1508 - 1542
lox2272
Type:protein_bind
Length:34 bp
Location:659 - 693
loxP
Type:protein_bind
Length:34 bp
Location:1592 - 1626
loxP
Type:protein_bind
Length:34 bp
Location:743 - 777
Factor Xa site
Type:cds
Length:12 bp
Location:2121 - 2133
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:2786 - 2927
WPRE
Type:other
Length:589 bp
Location:1650 - 2239
hGH poly(A) signal
Type:polya_signal
Length:477 bp
Location:2270 - 2747
hSyn promoter
Type:promoter
Length:448 bp
Location:174 - 622
mCherry
Type:cds
Length:711 bp
Location:785 - 1496
Kozak sequence
Type:regulatory
Length:10 bp
Location:1492 - 1502
f1 ori
Type:origin
Length:456 bp
Location:3001 - 3457
ori
Type:origin
Length:589 bp
Location:4874 - 5463
AmpR
Type:cds
Length:861 bp
Location:3843 - 4704
AmpR promoter
Type:promoter
Length:105 bp
Location:3738 - 3843
AAV2 ITR (alternate)
Type:repeat_region
Length:130 bp
Location:0 - 130
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:2786 - 2916
Amp-R
Type:primer_bind
Length:20 bp
Location:4060 - 4080
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:3297 - 3319
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:5214 - 5224
F1ori-R
Type:primer_bind
Length:20 bp
Location:3087 - 3107
hGH-PA-R
Type:primer_bind
Length:20 bp
Location:2468 - 2488
mCherry-F
Type:primer_bind
Length:20 bp
Location:1062 - 1082
mCherry-R
Type:primer_bind
Length:19 bp
Location:1331 - 1350
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:5362 - 5382
pBRforEco
Type:primer_bind
Length:19 bp
Location:3651 - 3670
pGEX 3'
Type:primer_bind
Length:23 bp
Location:3591 - 3614
pRS-marker
Type:primer_bind
Length:20 bp
Location:3472 - 3492
WPRE-R
Type:primer_bind
Length:21 bp
Location:1702 - 1723
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
lox2272 | protein_bind | → right | 1508 - 1542 | 34 bp | |
lox2272 | protein_bind | ← left | 659 - 693 | 34 bp | |
loxP | protein_bind | → right | 1592 - 1626 | 34 bp | |
loxP | protein_bind | ← left | 743 - 777 | 34 bp | |
Factor Xa site | cds | ← left | 2121 - 2133 | 12 bp | |
AAV2 ITR | repeat_region | → right | 2786 - 2927 | 141 bp | |
WPRE | other | → right | 1650 - 2239 | 589 bp | |
hGH poly(A) signal | polya_signal | → right | 2270 - 2747 | 477 bp | |
hSyn promoter | promoter | → right | 174 - 622 | 448 bp | |
mCherry | cds | ← left | 785 - 1496 | 711 bp | |
Kozak sequence | regulatory | → right | 1492 - 1502 | 10 bp | |
f1 ori | origin | → right | 3001 - 3457 | 456 bp | |
ori | origin | → right | 4874 - 5463 | 589 bp | |
AmpR | cds | → right | 3843 - 4704 | 861 bp | |
AmpR promoter | promoter | → right | 3738 - 3843 | 105 bp | |
AAV2 ITR (alternate) | repeat_region | → right | 0 - 130 | 130 bp | |
AAV2 ITR | repeat_region | → right | 2786 - 2916 | 130 bp | |
Amp-R | primer_bind | ← left | 4060 - 4080 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 3297 - 3319 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 5214 - 5224 | 10 bp | |
F1ori-R | primer_bind | ← left | 3087 - 3107 | 20 bp | |
hGH-PA-R | primer_bind | ← left | 2468 - 2488 | 20 bp | |
mCherry-F | primer_bind | ← left | 1062 - 1082 | 20 bp | |
mCherry-R | primer_bind | → right | 1331 - 1350 | 19 bp | |
pBR322ori-F | primer_bind | → right | 5362 - 5382 | 20 bp | |
pBRforEco | primer_bind | ← left | 3651 - 3670 | 19 bp | |
pGEX 3' | primer_bind | → right | 3591 - 3614 | 23 bp | |
pRS-marker | primer_bind | ← left | 3472 - 3492 | 20 bp | |
WPRE-R | primer_bind | ← left | 1702 - 1723 | 21 bp |