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Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pAAV-hSyn-EGFP
5265 bp
All features (27)
Factor Xa site
Type:cds
Length:12 bp
Location:1867 - 1879
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:2532 - 2673
WPRE
Type:other
Length:589 bp
Location:1396 - 1985
hGH poly(A) signal
Type:polya_signal
Length:477 bp
Location:2016 - 2493
hSyn promoter
Type:promoter
Length:448 bp
Location:179 - 627
EGFP
Type:cds
Length:717 bp
Location:652 - 1369
EGFP
Type:cds
Length:720 bp
Location:652 - 1372
Kozak sequence
Type:regulatory
Length:10 bp
Location:646 - 656
f1 ori
Type:origin
Length:456 bp
Location:2747 - 3203
ori
Type:origin
Length:589 bp
Location:4620 - 5209
AmpR
Type:cds
Length:861 bp
Location:3589 - 4450
AmpR promoter
Type:promoter
Length:105 bp
Location:3484 - 3589
AAV2 ITR (alternate)
Type:repeat_region
Length:130 bp
Location:5 - 135
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:2532 - 2662
Amp-R
Type:primer_bind
Length:20 bp
Location:3806 - 3826
EGFP-C
Type:primer_bind
Length:22 bp
Location:1304 - 1326
EGFP-N
Type:primer_bind
Length:22 bp
Location:696 - 718
EXFP-R
Type:primer_bind
Length:20 bp
Location:957 - 977
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:3043 - 3065
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:4960 - 4970
F1ori-R
Type:primer_bind
Length:20 bp
Location:2833 - 2853
hGH-PA-R
Type:primer_bind
Length:20 bp
Location:2214 - 2234
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:5108 - 5128
pBRforEco
Type:primer_bind
Length:19 bp
Location:3397 - 3416
pGEX 3'
Type:primer_bind
Length:23 bp
Location:3337 - 3360
pRS-marker
Type:primer_bind
Length:20 bp
Location:3218 - 3238
WPRE-R
Type:primer_bind
Length:21 bp
Location:1448 - 1469
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
Factor Xa site | cds | ← left | 1867 - 1879 | 12 bp | |
AAV2 ITR | repeat_region | → right | 2532 - 2673 | 141 bp | |
WPRE | other | → right | 1396 - 1985 | 589 bp | |
hGH poly(A) signal | polya_signal | → right | 2016 - 2493 | 477 bp | |
hSyn promoter | promoter | → right | 179 - 627 | 448 bp | |
EGFP | cds | → right | 652 - 1369 | 717 bp | |
EGFP | cds | → right | 652 - 1372 | 720 bp | |
Kozak sequence | regulatory | → right | 646 - 656 | 10 bp | |
f1 ori | origin | → right | 2747 - 3203 | 456 bp | |
ori | origin | → right | 4620 - 5209 | 589 bp | |
AmpR | cds | → right | 3589 - 4450 | 861 bp | |
AmpR promoter | promoter | → right | 3484 - 3589 | 105 bp | |
AAV2 ITR (alternate) | repeat_region | → right | 5 - 135 | 130 bp | |
AAV2 ITR | repeat_region | → right | 2532 - 2662 | 130 bp | |
Amp-R | primer_bind | ← left | 3806 - 3826 | 20 bp | |
EGFP-C | primer_bind | → right | 1304 - 1326 | 22 bp | |
EGFP-N | primer_bind | ← left | 696 - 718 | 22 bp | |
EXFP-R | primer_bind | ← left | 957 - 977 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 3043 - 3065 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 4960 - 4970 | 10 bp | |
F1ori-R | primer_bind | ← left | 2833 - 2853 | 20 bp | |
hGH-PA-R | primer_bind | ← left | 2214 - 2234 | 20 bp | |
pBR322ori-F | primer_bind | → right | 5108 - 5128 | 20 bp | |
pBRforEco | primer_bind | ← left | 3397 - 3416 | 19 bp | |
pGEX 3' | primer_bind | → right | 3337 - 3360 | 23 bp | |
pRS-marker | primer_bind | ← left | 3218 - 3238 | 20 bp | |
WPRE-R | primer_bind | ← left | 1448 - 1469 | 21 bp |