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Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pLenti CMV GFP Puro (658-5)
8619 bp
All features (59)
M13 rev
Type:primer_bind
Length:17 bp
Location:2135 - 2152
M13 fwd
Type:primer_bind
Length:17 bp
Location:8005 - 8022
Factor Xa site
Type:cds
Length:12 bp
Location:5967 - 5979
gp41 peptide
Type:cds
Length:45 bp
Location:3708 - 3753
cPPT/CTS
Type:other
Length:117 bp
Location:4019 - 4136
RRE
Type:other
Length:234 bp
Location:3290 - 3524
HIV-1 Ψ
Type:other
Length:126 bp
Location:2672 - 2798
3' LTR (ΔU3)
Type:ltr
Length:234 bp
Location:7355 - 7589
5' LTR (truncated)
Type:ltr
Length:181 bp
Location:2445 - 2626
WPRE
Type:other
Length:589 bp
Location:5496 - 6085
PGK promoter
Type:promoter
Length:500 bp
Location:6098 - 6598
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:7660 - 7782
SV40 ori
Type:origin
Length:136 bp
Location:7821 - 7957
CMV promoter
Type:promoter
Length:204 bp
Location:4462 - 4666
CMV enhancer
Type:enhancer
Length:304 bp
Location:4158 - 4462
RSV promoter
Type:promoter
Length:227 bp
Location:2218 - 2445
EGFP
Type:cds
Length:717 bp
Location:4761 - 5478
EGFP
Type:cds
Length:720 bp
Location:4761 - 5481
T3 promoter
Type:promoter
Length:19 bp
Location:2172 - 2191
T7 promoter
Type:promoter
Length:19 bp
Location:7977 - 7996
Kozak sequence
Type:regulatory
Length:10 bp
Location:4755 - 4765
f1 ori
Type:origin
Length:429 bp
Location:8163 - 8592
ori
Type:origin
Length:589 bp
Location:1161 - 1750
lac operator
Type:protein_bind
Length:17 bp
Location:2111 - 2128
CAP binding site
Type:protein_bind
Length:22 bp
Location:2037 - 2059
lac promoter
Type:promoter
Length:31 bp
Location:2073 - 2104
PuroR
Type:cds
Length:600 bp
Location:6618 - 7218
AmpR
Type:cds
Length:861 bp
Location:130 - 991
AmpR promoter
Type:promoter
Length:105 bp
Location:25 - 130
Amp-R
Type:primer_bind
Length:20 bp
Location:347 - 367
CMV-F
Type:primer_bind
Length:21 bp
Location:4615 - 4636
EBV-rev
Type:primer_bind
Length:20 bp
Location:7750 - 7770
EGFP-C
Type:primer_bind
Length:22 bp
Location:5413 - 5435
EGFP-N
Type:primer_bind
Length:22 bp
Location:4805 - 4827
EXFP-R
Type:primer_bind
Length:20 bp
Location:5066 - 5086
F1ori-F (site 1)
Type:primer_bind
Length:10 bp
Location:1501 - 1511
F1ori-F (site 2)
Type:primer_bind
Length:22 bp
Location:8459 - 8481
F1ori-R
Type:primer_bind
Length:20 bp
Location:8249 - 8269
L4440
Type:primer_bind
Length:18 bp
Location:1902 - 1920
LNCX
Type:primer_bind
Length:25 bp
Location:4661 - 4686
M13 Forward
Type:primer_bind
Length:18 bp
Location:8004 - 8022
M13 Reverse
Type:primer_bind
Length:17 bp
Location:2134 - 2151
M13/pUC Forward
Type:primer_bind
Length:23 bp
Location:8013 - 8036
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:2115 - 2138
mPGK-F
Type:primer_bind
Length:20 bp
Location:6529 - 6549
MSCV-rev
Type:primer_bind
Length:24 bp
Location:6132 - 6156
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:1649 - 1669
Puro-F
Type:primer_bind
Length:21 bp
Location:7113 - 7134
Puro-R (site 1)
Type:primer_bind
Length:20 bp
Location:6617 - 6637
Puro-R (site 2)
Type:primer_bind
Length:10 bp
Location:6899 - 6909
SV40pA-R (site 1)
Type:primer_bind
Length:11 bp
Location:3427 - 3438
SV40pA-R (site 2)
Type:primer_bind
Length:10 bp
Location:5598 - 5608
SV40pA-R (site 3)
Type:primer_bind
Length:20 bp
Location:7696 - 7716
T3 (site 1)
Type:primer_bind
Length:21 bp
Location:2169 - 2190
T3 (site 2)
Type:primer_bind
Length:11 bp
Location:7976 - 7987
T7 (site 1)
Type:primer_bind
Length:12 bp
Location:2179 - 2191
T7 (site 2)
Type:primer_bind
Length:20 bp
Location:7975 - 7995
WPRE-R (site 1)
Type:primer_bind
Length:12 bp
Location:3505 - 3517
WPRE-R (site 2)
Type:primer_bind
Length:21 bp
Location:5548 - 5569
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | → right | 2135 - 2152 | 17 bp | |
M13 fwd | primer_bind | ← left | 8005 - 8022 | 17 bp | |
Factor Xa site | cds | ← left | 5967 - 5979 | 12 bp | |
gp41 peptide | cds | → right | 3708 - 3753 | 45 bp | |
cPPT/CTS | other | → right | 4019 - 4136 | 117 bp | |
RRE | other | → right | 3290 - 3524 | 234 bp | |
HIV-1 Ψ | other | → right | 2672 - 2798 | 126 bp | |
3' LTR (ΔU3) | ltr | → right | 7355 - 7589 | 234 bp | |
5' LTR (truncated) | ltr | → right | 2445 - 2626 | 181 bp | |
WPRE | other | → right | 5496 - 6085 | 589 bp | |
PGK promoter | promoter | → right | 6098 - 6598 | 500 bp | |
SV40 poly(A) signal | polya_signal | → right | 7660 - 7782 | 122 bp | |
SV40 ori | origin | → right | 7821 - 7957 | 136 bp | |
CMV promoter | promoter | → right | 4462 - 4666 | 204 bp | |
CMV enhancer | enhancer | → right | 4158 - 4462 | 304 bp | |
RSV promoter | promoter | → right | 2218 - 2445 | 227 bp | |
EGFP | cds | → right | 4761 - 5478 | 717 bp | |
EGFP | cds | → right | 4761 - 5481 | 720 bp | |
T3 promoter | promoter | → right | 2172 - 2191 | 19 bp | |
T7 promoter | promoter | ← left | 7977 - 7996 | 19 bp | |
Kozak sequence | regulatory | → right | 4755 - 4765 | 10 bp | |
f1 ori | origin | → right | 8163 - 8592 | 429 bp | |
ori | origin | → right | 1161 - 1750 | 589 bp | |
lac operator | protein_bind | → right | 2111 - 2128 | 17 bp | |
CAP binding site | protein_bind | → right | 2037 - 2059 | 22 bp | |
lac promoter | promoter | → right | 2073 - 2104 | 31 bp | |
PuroR | cds | → right | 6618 - 7218 | 600 bp | |
AmpR | cds | → right | 130 - 991 | 861 bp | |
AmpR promoter | promoter | → right | 25 - 130 | 105 bp | |
Amp-R | primer_bind | ← left | 347 - 367 | 20 bp | |
CMV-F | primer_bind | → right | 4615 - 4636 | 21 bp | |
EBV-rev | primer_bind | → right | 7750 - 7770 | 20 bp | |
EGFP-C | primer_bind | → right | 5413 - 5435 | 22 bp | |
EGFP-N | primer_bind | ← left | 4805 - 4827 | 22 bp | |
EXFP-R | primer_bind | ← left | 5066 - 5086 | 20 bp | |
F1ori-F (site 1) | primer_bind | ← left | 1501 - 1511 | 10 bp | |
F1ori-F (site 2) | primer_bind | → right | 8459 - 8481 | 22 bp | |
F1ori-R | primer_bind | ← left | 8249 - 8269 | 20 bp | |
L4440 | primer_bind | → right | 1902 - 1920 | 18 bp | |
LNCX | primer_bind | → right | 4661 - 4686 | 25 bp | |
M13 Forward | primer_bind | ← left | 8004 - 8022 | 18 bp | |
M13 Reverse | primer_bind | → right | 2134 - 2151 | 17 bp | |
M13/pUC Forward | primer_bind | ← left | 8013 - 8036 | 23 bp | |
M13/pUC Reverse | primer_bind | → right | 2115 - 2138 | 23 bp | |
mPGK-F | primer_bind | → right | 6529 - 6549 | 20 bp | |
MSCV-rev | primer_bind | ← left | 6132 - 6156 | 24 bp | |
pBR322ori-F | primer_bind | → right | 1649 - 1669 | 20 bp | |
Puro-F | primer_bind | → right | 7113 - 7134 | 21 bp | |
Puro-R (site 1) | primer_bind | ← left | 6617 - 6637 | 20 bp | |
Puro-R (site 2) | primer_bind | ← left | 6899 - 6909 | 10 bp | |
SV40pA-R (site 1) | primer_bind | ← left | 3427 - 3438 | 11 bp | |
SV40pA-R (site 2) | primer_bind | → right | 5598 - 5608 | 10 bp | |
SV40pA-R (site 3) | primer_bind | ← left | 7696 - 7716 | 20 bp | |
T3 (site 1) | primer_bind | → right | 2169 - 2190 | 21 bp | |
T3 (site 2) | primer_bind | ← left | 7976 - 7987 | 11 bp | |
T7 (site 1) | primer_bind | → right | 2179 - 2191 | 12 bp | |
T7 (site 2) | primer_bind | ← left | 7975 - 7995 | 20 bp | |
WPRE-R (site 1) | primer_bind | → right | 3505 - 3517 | 12 bp | |
WPRE-R (site 2) | primer_bind | ← left | 5548 - 5569 | 21 bp |