Files
t
A
Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pSpCas9(BB)-2A-GFP (PX458)
9288 bp
All features (36)
T2A
Type:cds
Length:54 bp
Location:889 - 943
3xFLAG
Type:cds
Length:66 bp
Location:5896 - 5962
AAV2 ITR
Type:repeat_region
Length:141 bp
Location:1906 - 2047
U6 promoter
Type:promoter
Length:249 bp
Location:4644 - 4893
bGH poly(A) signal
Type:polya_signal
Length:208 bp
Location:1690 - 1898
hybrid intron
Type:intron
Length:228 bp
Location:5648 - 5876
SV40 NLS
Type:cds
Length:21 bp
Location:5968 - 5989
nucleoplasmin NLS
Type:cds
Length:48 bp
Location:826 - 874
chicken β-actin promoter
Type:promoter
Length:278 bp
Location:5370 - 5648
CMV enhancer
Type:enhancer
Length:286 bp
Location:5083 - 5369
EGFP
Type:cds
Length:714 bp
Location:943 - 1657
Kozak sequence
Type:regulatory
Length:10 bp
Location:5887 - 5897
f1 ori
Type:origin
Length:456 bp
Location:2121 - 2577
ori
Type:origin
Length:589 bp
Location:3994 - 4583
gRNA scaffold
Type:misc_rna
Length:76 bp
Location:4911 - 4987
Cas9
Type:cds
Length:-5187 bp
Location:6013 - 826
AmpR
Type:cds
Length:861 bp
Location:2963 - 3824
AmpR promoter
Type:promoter
Length:105 bp
Location:2858 - 2963
AAV2 ITR
Type:repeat_region
Length:130 bp
Location:1906 - 2036
Amp-R
Type:primer_bind
Length:20 bp
Location:3180 - 3200
BGH-rev (site 1)
Type:primer_bind
Length:18 bp
Location:1683 - 1701
BGH-rev (site 2)
Type:primer_bind
Length:16 bp
Location:6174 - 6190
EGFP-C (site 1)
Type:primer_bind
Length:22 bp
Location:1592 - 1614
EGFP-C (site 2)
Type:primer_bind
Length:10 bp
Location:9218 - 9228
EXFP-R (site 1)
Type:primer_bind
Length:20 bp
Location:1245 - 1265
EXFP-R (site 2)
Type:primer_bind
Length:10 bp
Location:7224 - 7234
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:2417 - 2439
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:4334 - 4344
F1ori-F (site 3)
Type:primer_bind
Length:10 bp
Location:8886 - 8896
F1ori-R
Type:primer_bind
Length:20 bp
Location:2207 - 2227
hU6-F
Type:primer_bind
Length:21 bp
Location:4643 - 4664
LKO.1 5'
Type:primer_bind
Length:20 bp
Location:4814 - 4834
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:4482 - 4502
pBRforEco
Type:primer_bind
Length:19 bp
Location:2771 - 2790
pGEX 3'
Type:primer_bind
Length:23 bp
Location:2711 - 2734
pRS-marker
Type:primer_bind
Length:20 bp
Location:2592 - 2612
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
T2A | cds | → right | 889 - 943 | 54 bp | |
3xFLAG | cds | → right | 5896 - 5962 | 66 bp | |
AAV2 ITR | repeat_region | → right | 1906 - 2047 | 141 bp | |
U6 promoter | promoter | → right | 4644 - 4893 | 249 bp | |
bGH poly(A) signal | polya_signal | → right | 1690 - 1898 | 208 bp | |
hybrid intron | intron | → right | 5648 - 5876 | 228 bp | |
SV40 NLS | cds | → right | 5968 - 5989 | 21 bp | |
nucleoplasmin NLS | cds | → right | 826 - 874 | 48 bp | |
chicken β-actin promoter | promoter | → right | 5370 - 5648 | 278 bp | |
CMV enhancer | enhancer | → right | 5083 - 5369 | 286 bp | |
EGFP | cds | → right | 943 - 1657 | 714 bp | |
Kozak sequence | regulatory | → right | 5887 - 5897 | 10 bp | |
f1 ori | origin | → right | 2121 - 2577 | 456 bp | |
ori | origin | → right | 3994 - 4583 | 589 bp | |
gRNA scaffold | misc_rna | → right | 4911 - 4987 | 76 bp | |
Cas9 | cds | → right | 6013 - 826 | -5187 bp | |
AmpR | cds | → right | 2963 - 3824 | 861 bp | |
AmpR promoter | promoter | → right | 2858 - 2963 | 105 bp | |
AAV2 ITR | repeat_region | → right | 1906 - 2036 | 130 bp | |
Amp-R | primer_bind | ← left | 3180 - 3200 | 20 bp | |
BGH-rev (site 1) | primer_bind | ← left | 1683 - 1701 | 18 bp | |
BGH-rev (site 2) | primer_bind | → right | 6174 - 6190 | 16 bp | |
EGFP-C (site 1) | primer_bind | → right | 1592 - 1614 | 22 bp | |
EGFP-C (site 2) | primer_bind | → right | 9218 - 9228 | 10 bp | |
EXFP-R (site 1) | primer_bind | ← left | 1245 - 1265 | 20 bp | |
EXFP-R (site 2) | primer_bind | ← left | 7224 - 7234 | 10 bp | |
F1ori-F (site 1) | primer_bind | → right | 2417 - 2439 | 22 bp | |
F1ori-F (site 2) | primer_bind | ← left | 4334 - 4344 | 10 bp | |
F1ori-F (site 3) | primer_bind | → right | 8886 - 8896 | 10 bp | |
F1ori-R | primer_bind | ← left | 2207 - 2227 | 20 bp | |
hU6-F | primer_bind | → right | 4643 - 4664 | 21 bp | |
LKO.1 5' | primer_bind | → right | 4814 - 4834 | 20 bp | |
pBR322ori-F | primer_bind | → right | 4482 - 4502 | 20 bp | |
pBRforEco | primer_bind | ← left | 2771 - 2790 | 19 bp | |
pGEX 3' | primer_bind | → right | 2711 - 2734 | 23 bp | |
pRS-marker | primer_bind | ← left | 2592 - 2612 | 20 bp |