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Amir SaberiUpdate construct names and descriptions
42dc991•1 month ago
pcDNA3-EGFP
6159 bp
All features (45)
M13 rev
Type:primer_bind
Length:17 bp
Location:3767 - 3784
bGH poly(A) signal
Type:polya_signal
Length:225 bp
Location:1521 - 1746
SV40 poly(A) signal
Type:polya_signal
Length:122 bp
Location:3597 - 3719
SV40 ori
Type:origin
Length:136 bp
Location:2413 - 2549
SV40 promoter
Type:promoter
Length:330 bp
Location:2233 - 2563
CMV promoter
Type:promoter
Length:204 bp
Location:380 - 584
CMV enhancer
Type:enhancer
Length:380 bp
Location:0 - 380
EGFP
Type:cds
Length:717 bp
Location:742 - 1459
EGFP
Type:cds
Length:720 bp
Location:742 - 1462
SP6 promoter
Type:promoter
Length:19 bp
Location:1477 - 1496
T7 promoter
Type:promoter
Length:19 bp
Location:628 - 647
f1 ori
Type:origin
Length:429 bp
Location:1791 - 2220
ori
Type:origin
Length:589 bp
Location:4169 - 4758
lac operator
Type:protein_bind
Length:17 bp
Location:3791 - 3808
CAP binding site
Type:protein_bind
Length:22 bp
Location:3860 - 3882
lac promoter
Type:promoter
Length:31 bp
Location:3815 - 3846
NeoR/KanR
Type:cds
Length:795 bp
Location:2629 - 3424
AmpR
Type:cds
Length:861 bp
Location:4928 - 5789
AmpR promoter
Type:promoter
Length:105 bp
Location:5789 - 5894
Amp-R
Type:primer_bind
Length:20 bp
Location:5550 - 5570
BGH-rev
Type:primer_bind
Length:18 bp
Location:1514 - 1532
CMV-F
Type:primer_bind
Length:21 bp
Location:533 - 554
EBV-rev
Type:primer_bind
Length:20 bp
Location:3687 - 3707
EGFP-C
Type:primer_bind
Length:22 bp
Location:1394 - 1416
EGFP-N
Type:primer_bind
Length:22 bp
Location:786 - 808
EXFP-R
Type:primer_bind
Length:20 bp
Location:1047 - 1067
F1ori-F (site 1)
Type:primer_bind
Length:22 bp
Location:2087 - 2109
F1ori-F (site 2)
Type:primer_bind
Length:10 bp
Location:4406 - 4416
F1ori-R
Type:primer_bind
Length:20 bp
Location:1877 - 1897
L4440 (site 1)
Type:primer_bind
Length:18 bp
Location:3997 - 4015
L4440 (site 2)
Type:primer_bind
Length:10 bp
Location:6038 - 6048
M13 Reverse
Type:primer_bind
Length:17 bp
Location:3766 - 3783
M13/pUC Reverse
Type:primer_bind
Length:23 bp
Location:3779 - 3802
Neo-F
Type:primer_bind
Length:20 bp
Location:3292 - 3312
Neo-R
Type:primer_bind
Length:20 bp
Location:2682 - 2702
pBABE 3'
Type:primer_bind
Length:21 bp
Location:2227 - 2248
pBR322ori-F
Type:primer_bind
Length:20 bp
Location:4248 - 4268
pRS-marker
Type:primer_bind
Length:20 bp
Location:5967 - 5987
SP6
Type:primer_bind
Length:18 bp
Location:1477 - 1495
SV40pA-R
Type:primer_bind
Length:20 bp
Location:3633 - 3653
SV40pro-F (site 1)
Type:primer_bind
Length:10 bp
Location:837 - 847
SV40pro-F (site 2)
Type:primer_bind
Length:20 bp
Location:2474 - 2494
SV40pro-F (site 3)
Type:primer_bind
Length:12 bp
Location:2497 - 2509
SV40pro-F (site 4)
Type:primer_bind
Length:10 bp
Location:2542 - 2552
T7
Type:primer_bind
Length:20 bp
Location:627 - 647
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
M13 rev | primer_bind | ← left | 3767 - 3784 | 17 bp | |
bGH poly(A) signal | polya_signal | → right | 1521 - 1746 | 225 bp | |
SV40 poly(A) signal | polya_signal | → right | 3597 - 3719 | 122 bp | |
SV40 ori | origin | → right | 2413 - 2549 | 136 bp | |
SV40 promoter | promoter | → right | 2233 - 2563 | 330 bp | |
CMV promoter | promoter | → right | 380 - 584 | 204 bp | |
CMV enhancer | enhancer | → right | 0 - 380 | 380 bp | |
EGFP | cds | → right | 742 - 1459 | 717 bp | |
EGFP | cds | → right | 742 - 1462 | 720 bp | |
SP6 promoter | promoter | ← left | 1477 - 1496 | 19 bp | |
T7 promoter | promoter | → right | 628 - 647 | 19 bp | |
f1 ori | origin | → right | 1791 - 2220 | 429 bp | |
ori | origin | ← left | 4169 - 4758 | 589 bp | |
lac operator | protein_bind | → right | 3791 - 3808 | 17 bp | |
CAP binding site | protein_bind | → right | 3860 - 3882 | 22 bp | |
lac promoter | promoter | ← left | 3815 - 3846 | 31 bp | |
NeoR/KanR | cds | → right | 2629 - 3424 | 795 bp | |
AmpR | cds | ← left | 4928 - 5789 | 861 bp | |
AmpR promoter | promoter | ← left | 5789 - 5894 | 105 bp | |
Amp-R | primer_bind | → right | 5550 - 5570 | 20 bp | |
BGH-rev | primer_bind | ← left | 1514 - 1532 | 18 bp | |
CMV-F | primer_bind | → right | 533 - 554 | 21 bp | |
EBV-rev | primer_bind | → right | 3687 - 3707 | 20 bp | |
EGFP-C | primer_bind | → right | 1394 - 1416 | 22 bp | |
EGFP-N | primer_bind | ← left | 786 - 808 | 22 bp | |
EXFP-R | primer_bind | ← left | 1047 - 1067 | 20 bp | |
F1ori-F (site 1) | primer_bind | → right | 2087 - 2109 | 22 bp | |
F1ori-F (site 2) | primer_bind | → right | 4406 - 4416 | 10 bp | |
F1ori-R | primer_bind | ← left | 1877 - 1897 | 20 bp | |
L4440 (site 1) | primer_bind | ← left | 3997 - 4015 | 18 bp | |
L4440 (site 2) | primer_bind | → right | 6038 - 6048 | 10 bp | |
M13 Reverse | primer_bind | ← left | 3766 - 3783 | 17 bp | |
M13/pUC Reverse | primer_bind | ← left | 3779 - 3802 | 23 bp | |
Neo-F | primer_bind | → right | 3292 - 3312 | 20 bp | |
Neo-R | primer_bind | ← left | 2682 - 2702 | 20 bp | |
pBABE 3' | primer_bind | ← left | 2227 - 2248 | 21 bp | |
pBR322ori-F | primer_bind | ← left | 4248 - 4268 | 20 bp | |
pRS-marker | primer_bind | ← left | 5967 - 5987 | 20 bp | |
SP6 | primer_bind | ← left | 1477 - 1495 | 18 bp | |
SV40pA-R | primer_bind | ← left | 3633 - 3653 | 20 bp | |
SV40pro-F (site 1) | primer_bind | → right | 837 - 847 | 10 bp | |
SV40pro-F (site 2) | primer_bind | → right | 2474 - 2494 | 20 bp | |
SV40pro-F (site 3) | primer_bind | ← left | 2497 - 2509 | 12 bp | |
SV40pro-F (site 4) | primer_bind | → right | 2542 - 2552 | 10 bp | |
T7 | primer_bind | → right | 627 - 647 | 20 bp |