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Amir SaberiAdd CasMINI reference expression construct
8313b3f•2 days ago
CAG_CasMINI_U6_gRNA_human_expression
2875 bp
All features (7)
hU6
Type:promoter
Length:248 bp
Location:0 - 248
CMV enhancer
Type:enhancer
Length:285 bp
Location:445 - 730
chicken β-actin promoter
Type:promoter
Length:256 bp
Location:732 - 988
hybrid intron
Type:intron
Length:227 bp
Location:989 - 1216
Kozak
Type:miscellaneous
Length:5 bp
Location:1228 - 1233
CasMINI
Type:cds
Length:1640 bp
Location:1234 - 2874
SV40-NLS
Type:tag
Length:20 bp
Location:1240 - 1260
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
hU6 | promoter | → right | 0 - 248 | 248 bp | |
CMV enhancer | enhancer | → right | 445 - 730 | 285 bp | |
chicken β-actin promoter | promoter | → right | 732 - 988 | 256 bp | |
hybrid intron | intron | → right | 989 - 1216 | 227 bp | |
Kozak | miscellaneous | → right | 1228 - 1233 | 5 bp | |
CasMINI | cds | → right | 1234 - 2874 | 1640 bp | |
SV40-NLS | tag | → right | 1240 - 1260 | 20 bp |