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Amir SaberiAdd SaCas9 reference nuclease and AAV construct
ceb67f6•2 days ago
AAV_SaCas9_human_codon_optimized
7446 bp
All features (27)
AAV2 ITR (alternate)
Type:repeat_region
Length:129 bp
Location:0 - 129
CMV enhancer
Type:enhancer
Length:379 bp
Location:153 - 532
CMV promoter
Type:promoter
Length:203 bp
Location:533 - 736
CMV-F_1
Type:primer_bind
Length:20 bp
Location:687 - 707
Kozak sequence
Type:regulatory
Length:9 bp
Location:755 - 764
SV40 NLS
Type:cds
Length:20 bp
Location:767 - 787
SaCas9
Type:cds
Length:3155 bp
Location:812 - 3967
nucleoplasmin NLS
Type:cds
Length:47 bp
Location:3968 - 4015
3xHA
Type:cds
Length:80 bp
Location:4022 - 4102
BGH-rev_1
Type:primer_bind
Length:17 bp
Location:4130 - 4147
bGH poly-A signal
Type:polya_signal
Length:207 bp
Location:4136 - 4343
U6 promoter
Type:promoter
Length:248 bp
Location:4350 - 4598
hU6-F_1
Type:primer_bind
Length:20 bp
Location:4350 - 4370
LKO.1 5'_1
Type:primer_bind
Length:19 bp
Location:4521 - 4540
Sa gRNA scaffold
Type:misc_rna
Length:75 bp
Location:4619 - 4694
AAV2 ITR
Type:repeat_region
Length:129 bp
Location:4708 - 4837
f1 ori
Type:rep_origin
Length:455 bp
Location:4923 - 5378
F1ori-R_1
Type:primer_bind
Length:19 bp
Location:5010 - 5029
F1ori-F_2
Type:primer_bind
Length:21 bp
Location:5220 - 5241
pRS-marker_2
Type:primer_bind
Length:19 bp
Location:5395 - 5414
pGEX 3'_1
Type:primer_bind
Length:22 bp
Location:5514 - 5536
pBRforEco_1
Type:primer_bind
Length:18 bp
Location:5574 - 5592
AmpR promoter
Type:promoter
Length:104 bp
Location:5660 - 5764
AmpR
Type:cds
Length:860 bp
Location:5765 - 6625
Amp-R_1
Type:primer_bind
Length:19 bp
Location:5983 - 6002
ori
Type:rep_origin
Length:588 bp
Location:6796 - 7384
pBR322ori-F_1
Type:primer_bind
Length:19 bp
Location:7285 - 7304
| Color | Name | Type | Direction | Location | Length |
|---|---|---|---|---|---|
AAV2 ITR (alternate) | repeat_region | → right | 0 - 129 | 129 bp | |
CMV enhancer | enhancer | → right | 153 - 532 | 379 bp | |
CMV promoter | promoter | → right | 533 - 736 | 203 bp | |
CMV-F_1 | primer_bind | → right | 687 - 707 | 20 bp | |
Kozak sequence | regulatory | → right | 755 - 764 | 9 bp | |
SV40 NLS | cds | → right | 767 - 787 | 20 bp | |
SaCas9 | cds | → right | 812 - 3967 | 3155 bp | |
nucleoplasmin NLS | cds | → right | 3968 - 4015 | 47 bp | |
3xHA | cds | → right | 4022 - 4102 | 80 bp | |
BGH-rev_1 | primer_bind | ← left | 4130 - 4147 | 17 bp | |
bGH poly-A signal | polya_signal | → right | 4136 - 4343 | 207 bp | |
U6 promoter | promoter | → right | 4350 - 4598 | 248 bp | |
hU6-F_1 | primer_bind | → right | 4350 - 4370 | 20 bp | |
LKO.1 5'_1 | primer_bind | → right | 4521 - 4540 | 19 bp | |
Sa gRNA scaffold | misc_rna | → right | 4619 - 4694 | 75 bp | |
AAV2 ITR | repeat_region | → right | 4708 - 4837 | 129 bp | |
f1 ori | rep_origin | → right | 4923 - 5378 | 455 bp | |
F1ori-R_1 | primer_bind | ← left | 5010 - 5029 | 19 bp | |
F1ori-F_2 | primer_bind | → right | 5220 - 5241 | 21 bp | |
pRS-marker_2 | primer_bind | ← left | 5395 - 5414 | 19 bp | |
pGEX 3'_1 | primer_bind | → right | 5514 - 5536 | 22 bp | |
pBRforEco_1 | primer_bind | ← left | 5574 - 5592 | 18 bp | |
AmpR promoter | promoter | → right | 5660 - 5764 | 104 bp | |
AmpR | cds | → right | 5765 - 6625 | 860 bp | |
Amp-R_1 | primer_bind | ← left | 5983 - 6002 | 19 bp | |
ori | rep_origin | → right | 6796 - 7384 | 588 bp | |
pBR322ori-F_1 | primer_bind | → right | 7285 - 7304 | 19 bp |