Base Editing Systems

A curated collection of base editor plasmids for precise C-to-T and A-to-G genome editing without inducing double-strand DNA breaks.

What are base editors?

Base editors enable precise single-nucleotide changes in genomic DNA:

• Cytosine Base Editors (CBEs): Convert C•G to T•A
• Adenine Base Editors (ABEs): Convert A•T to G•C

Unlike traditional CRISPR/Cas9, base editors use catalytically impaired Cas proteins fused to deaminase enzymes, avoiding double-strand breaks and unwanted insertions/deletions.

What's in this repository

This collection includes base editors optimized for different applications:

Mammalian Expression Systems

• pCMV-BE2 - Foundational cytosine base editor (Liu lab, 2016)
• AID-BE3 - Improved CBE with higher efficiency
• pCMV_ABEmax_P2A_GFP - Optimized adenine base editor with GFP marker

AAV Delivery Systems

• pAAV-ABE-NT-sgRNA - Split ABE N-terminal half for dual-AAV delivery
• pAAV-ABE-CT - Split ABE C-terminal half (pairs with above)
• Cbh_v5 AAV-CBE C-terminal - Split CBE for in vivo tissue editing
• pEJS-HZ79 All-in-one AAV-NmeABE8e - Single AAV vector with compact Nme2Cas9-ABE
• pAAV-EFS-TadA8e-Sauri-U6-Lmna sgRNA - Therapeutic example targeting cardiac mutation

Advanced Variants

• pAAV-CASI-InteinC-NG - Split-intein system with relaxed PAM requirements

All sequences are from published and referenced research.

Key applications

• Disease modeling and correction of pathogenic mutations
• Generating precise point mutations for functional studies
• Creating stop codons or splice site variants
• In vivo gene therapy (via AAV delivery)

Custom base editing vectors

Need a custom base editor or AAV packaging? Order at Ambryon.com with:

• Custom guide RNA sequences
• Alternative promoters for tissue-specific expression
• Different AAV serotypes optimized for your target tissue
• Fully manufactured and quality-controlled AAV preps

Additional resources

• Addgene's base editing guide: https://www.addgene.org/crispr/base-edit/
• Original base editing publications linked in plasmid annotations