Reporter and Control Vectors

This repository contains well-characterized reporter constructs and controls for verifying transduction efficiency, measuring expression, and troubleshooting delivery methods.

What's included

Fluorescent Reporters

AAV-compatible:

• pAAV-CAG-GFP - Green fluorescent protein with strong constitutive CAG promoter
• pAAV-CAG-tdTomato - Red fluorescent protein, codon-optimized for mammalian expression
• pAAV-hSyn-EGFP - Neuron-specific expression with human synapsin promoter
• pAAV-hSyn-DIO-mCherry - Cre-dependent red reporter for conditional expression

Lentiviral:

• pLenti CMV GFP Puro - Third-generation lentiviral vector with puromycin selection

Plasmid:

• pcDNA3-EGFP - Mammalian expression plasmid for transfection

CRISPR with reporter:

• pSpCas9(BB)-2A-GFP (PX458) - CRISPR/Cas9 with GFP co-expression for tracking edited cells

Luciferase Reporters

Quantitative bioluminescent readouts for sensitive expression analysis:

• pAAV-CAG-FLuc - AAV-compatible firefly luciferase with CAG promoter
• Luciferase-pcDNA3 - Mammalian expression vector for firefly luciferase
• pGL4.18 CMV-Luc - Optimized firefly luciferase with CMV promoter

Common applications

• Test AAV serotype tropism - Use reporters to determine which serotype works best in your cell type before ordering custom AAV
• Optimize transfection conditions - Screen reagents, cell densities, and timing with fluorescent reporters
• Validate CRISPR delivery - PX458 combines Cas9 with GFP so you can track which cells received the editing machinery
• Dual-reporter assays - Combine firefly luciferase with Renilla for normalized measurements

Vector backbone variety

These constructs cover multiple delivery approaches:

• AAV transfer plasmids - ITR-flanked for AAV packaging (CAG-GFP, CAG-FLuc, hSyn-EGFP)
• Lentiviral vectors - For stable genomic integration (pLenti CMV GFP Puro)
• Standard plasmids - For transient transfection (pcDNA3-EGFP, Luciferase-pcDNA3)

Promoter options

• CAG - Strong constitutive expression in most cell types
• CMV - High expression in mammalian cells
• hSyn - Neuron-specific expression
• DIO (Cre-dependent) - Conditional expression

Need a custom reporter?

Visit Ambryon.com to order:

• Your gene of interest fused to any of these reporters
• Custom AAV packaging in 16+ serotypes (AAV1-9, PHP.eB, and more)
• Tissue-specific promoters optimized for your target cells
• Dual reporters for ratiometric or multicolor experiments

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Repository maintained by Ambryon